Harmonizing rhamnolipid biosurfactant production by halophilic Pseudomonas aeruginosa Pa84 by applying response surface methodology

IF 1.6 4区 工程技术 Q3 CHEMISTRY, APPLIED
Priyanka Sharma, Shantam Gautam, Siddhi Joshi, Mousumi Debnath
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Abstract

Biosurfactants are gaining attention due to their biobased nature, including reduced toxicity and enhanced biocompatibility. This research work investigates the characteristics of the biosurfactant extracted from the halophilic strain Pa84 of Pseudomonas aeruginosa and optimizes its production using a statistical model. The identity of biosurfactant from P. aeruginosa strain Pa84 as rhamnolipid was determined by comparing the genomic sequences of strain Pa84 with high-yielding strains of P. aeruginosa that produce rhamnolipid, through Fourier transform infrared and liquid chromatography-mass spectrometer analyses, and by the presence of rhlA, rhlB, and rhlC genes in the genome of strain Pa84 that are responsible for the production of rhamnolipid. A methodical strategy using a time-course assessment and statistical software optimized the production of rhamnolipids. After optimization using central composite design-response surface methodology, the medium composition of 3.34 g/L of NH4Cl, 1.03 g/L of NaCl, and 4 g/L of glycerol were chosen, yielding 7.48 ± 0.0217 g/L of biosurfactant. These findings will help in the development of effective and sustainable bioprocess that produce rhamnolipid biosurfactants, for use in a variety of sectors.

应用响应面法协调嗜盐性铜绿假单胞菌Pa84生产鼠李糖脂生物表面活性剂
生物表面活性剂由于其生物基性质,包括降低毒性和增强生物相容性而受到关注。本文研究了铜绿假单胞菌嗜盐菌株Pa84提取的生物表面活性剂的特性,并利用统计模型对其产量进行了优化。通过与产鼠李糖脂的铜绿假单胞菌Pa84的基因组序列比较、傅里叶变换红外和液相色谱-质谱分析,以及菌株Pa84基因组中产鼠李糖脂的rhlA、rhlB和rhlC基因的存在,确定了菌株Pa84的生物表面活性物质为鼠李糖脂。使用时间过程评估和统计软件的系统策略优化了鼠李糖脂的生产。采用中心复合设计-响应面法优化后,培养基组成为NH4Cl 3.34 g/L、NaCl 1.03 g/L、甘油4 g/L,产率为7.48±0.0217 g/L。这些发现将有助于开发有效和可持续的生产鼠李糖脂生物表面活性剂的生物工艺,用于各种部门。
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来源期刊
Journal of Surfactants and Detergents
Journal of Surfactants and Detergents 工程技术-工程:化工
CiteScore
3.80
自引率
6.20%
发文量
68
审稿时长
4 months
期刊介绍: Journal of Surfactants and Detergents, a journal of the American Oil Chemists’ Society (AOCS) publishes scientific contributions in the surfactants and detergents area. This includes the basic and applied science of petrochemical and oleochemical surfactants, the development and performance of surfactants in all applications, as well as the development and manufacture of detergent ingredients and their formulation into finished products.
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