Lectin-mediated adhesion: Testing of tailor-made cellulose derivatives with ConA and live E. coli bacteria

IF 3.3 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Carina Spormann , Tobias Dorn , Stefan Schwaiger , Sönke Sdunnus , Andreas Koschella , Hanspeter Kählig , Thomas Heinze , Thisbe K. Lindhorst , Tanja M. Wrodnigg
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Abstract

Due to the high exigence for treatment of bacterial infections, anti-adhesion therapy has emerged as an alternative method in an era of antibiotic resistance. Here, we have conjugated azido-functionalized cellulose with specific carbohydrate ligands (mannose and glucose, respectively) via copper-catalyzed azide-alkyne click chemistry (CuAAC) resulting in homo- and hetero-glycosylated cellulose derivatives with a DS > 0.9. The new materials, CellulMan and CellulManGlc, were evaluated as potential anti-adhesive reagents with type 1-fimbriated E. coli bacteria and additionally with the plant lectin Concanavalin A (ConA). We have determined the adhesion properties of the new glycopolymers as well as their agglutination behavior in an in-depth study combining two- and three-dimensional assays. The tested synthetic glycopolymers differ in their biological behavior as lectin ligands depending on the respective glycodecoration. In particular, CellulMan shows a remarkably low minimal effective concentration cmin ≤ 2 pg/mL as adhesive material with E. coli. Overall, the assays demonstrate that glyco-modified cellulose serves as an excellent antiadhesive as well as adhesive surface material for both type 1 fimbriated E. coli and ConA, being superior to natural polysaccharides.

Abstract Image

凝集素介导的粘附:用ConA和活大肠杆菌定制的纤维素衍生物的测试
由于治疗细菌感染的高需求,抗粘连治疗已成为抗生素耐药时代的一种替代方法。在这里,我们通过铜催化叠氮化物-炔键化学(CuAAC)将叠氮化物功能化纤维素与特定的碳水化合物配体(分别为甘露糖和葡萄糖)偶联,得到了具有DS和gt的同源和异糖基化纤维素衍生物;0.9. 新材料CellulMan和CellulManGlc分别作为1型缠绕大肠杆菌和植物凝集素ConA的潜在抗黏附试剂进行了评价。我们已经确定了新的糖共聚物的粘附性能,以及他们的凝集行为在一个深入的研究结合二维和三维分析。所测试的合成糖共聚物作为凝集素配体的生物学行为不同,取决于各自的糖修饰。特别是CellulMan作为大肠杆菌黏附材料的最低有效浓度(cmin≤2pg /mL)非常低。总的来说,实验表明,糖修饰纤维素作为1型纤维化大肠杆菌和ConA的优异的抗粘接和粘接表面材料,优于天然多糖。
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来源期刊
Bioorganic & Medicinal Chemistry
Bioorganic & Medicinal Chemistry 医学-生化与分子生物学
CiteScore
6.80
自引率
2.90%
发文量
413
审稿时长
17 days
期刊介绍: Bioorganic & Medicinal Chemistry provides an international forum for the publication of full original research papers and critical reviews on molecular interactions in key biological targets such as receptors, channels, enzymes, nucleotides, lipids and saccharides. The aim of the journal is to promote a better understanding at the molecular level of life processes, and living organisms, as well as the interaction of these with chemical agents. A special feature will be that colour illustrations will be reproduced at no charge to the author, provided that the Editor agrees that colour is essential to the information content of the illustration in question.
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