Camera-Based Measurement of Glucose Concentration in Aqueous Solution: Achieving High Precision and Accuracy with Frame Accumulation and Pixel Stacking

IF 1.5 4区 物理与天体物理 Q2 PHYSICS, MULTIDISCIPLINARY
Gang Li, Sonia Mustafa, Adnan Munawar, Ling Lin
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引用次数: 0

Abstract

Accurate measurement of glucose concentration in solution is imperative for diverse scientific applications. In this study, conducted at Tianjin University in 2024, the optical measurement accuracy of glucose in aqueous solution was substantially improved using a novel approach of pixel stacking and frame accumulation. Glucose solutions (n = 28) of different concentrations (range, 0.0–5.0%) were prepared, and the transmitted light intensity from these solutions was recorded using a polarization-sensitive camera. To improve the signal-to-noise ratio (SNR), we used frame accumulation and signal superposition of multiple pixels. The results showed a linear decrease in optical intensity captured by the camera with increasing glucose concentration in the solution. Following frame accumulation and pixel stacking, a substantial enhancement in precision and accuracy up to a factor of 1000 was achieved. Moreover, three algorithms including partial least squares (PLS) model, support vector machine (SVM) model, and random forest (RF) model were constructed and trained on one set of the samples and then used to blindly predict another set of the samples. The prediction of glucose concentration by the PLS model showed a minimum root mean square error (RMSE) of 0.2653, mean square error (MSE) of 0.0704, and a correlation coefficient of 0.9879 between the measured optical intensity and the glucose concentration. The use of frame accumulation and pixel stacking methods substantially improved glucose measurement accuracy.

基于相机的水溶液中葡萄糖浓度测量:通过帧积累和像素堆叠实现高精度和准确性
准确测量溶液中的葡萄糖浓度对于各种科学应用是必不可少的。在天津大学于2024年进行的这项研究中,采用了一种新的像素堆叠和帧积累方法,大大提高了水溶液中葡萄糖的光学测量精度。制备不同浓度的葡萄糖溶液(n = 28)(范围为0.0-5.0%),用偏光敏感相机记录这些溶液的透射光强。为了提高信噪比,我们采用了帧累加和多像素信号叠加的方法。结果表明,随着溶液中葡萄糖浓度的增加,相机捕捉到的光强呈线性下降。在帧积累和像素叠加之后,精度和准确度大幅提高,达到1000倍。构建偏最小二乘(PLS)模型、支持向量机(SVM)模型和随机森林(RF)模型三种算法,在一组样本上进行训练,然后用于盲预测另一组样本。PLS模型预测葡萄糖浓度的最小均方根误差(RMSE)为0.2653,均方误差(MSE)为0.0704,测量光强与葡萄糖浓度的相关系数为0.9879。使用帧积累和像素堆叠方法大大提高了葡萄糖测量精度。
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来源期刊
Brazilian Journal of Physics
Brazilian Journal of Physics 物理-物理:综合
CiteScore
2.50
自引率
6.20%
发文量
189
审稿时长
6.0 months
期刊介绍: The Brazilian Journal of Physics is a peer-reviewed international journal published by the Brazilian Physical Society (SBF). The journal publishes new and original research results from all areas of physics, obtained in Brazil and from anywhere else in the world. Contents include theoretical, practical and experimental papers as well as high-quality review papers. Submissions should follow the generally accepted structure for journal articles with basic elements: title, abstract, introduction, results, conclusions, and references.
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