Determination of paraoxonase activity and prooxidant-antioxidant balance in the brain tissue of rats following subacute administration of different K-oximes

IF 4.7 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chemico-Biological Interactions Pub Date : 2025-05-05 DOI:10.1016/j.cbi.2025.111539

Vesna Jaćević , Jelica Grujić-Milanović , Zoran Milovanović , Sladjan Milanović , Lana Nežić , Ljiljana Amidžić , Nataša Vojinović , Bojan Marković , Vladimir Dobričić , Petar Milosavljević , Eugenie Nepovimova , Kamil Kuča

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引用次数: 0

Abstract

This study aimed to determine the paraoxonase activity and prooxidant-antioxidant balance in the brain tissue of Wistar rats following subacute treatment with selected K-oximes. Each K-oxime was administered intramuscularly (0.1 LD₅₀/kg) twice per week for four weeks, and 7 days after the last treatment, the paraoxonase activity (PON1), the prooxidant-antioxidant balance (PAB), the levels of superoxide anion radical (O₂^•–), the concentration of nitrite (NO₂⁻) and the content of free protein thiol groups in the brain homogenates were evaluated. The PON1 and PAB activity were significantly reduced in almost all oxime-treated groups (p < 0.01 and p < 0.001, respectively). The concentrations of O₂^•– were significantly increased in the obidoxime-, K048-, K074- and K075-treated groups (p < 0.001), while the levels of NO₂⁻ was significantly decreased in asoxime-, obidoxime-, K074 and K075-treated rats (p < 0.01, p < 0.001, respectively). The content of Thiol groups was significantly elevated in all oxime-treated groups (p < 0.001). Continuing our previously published data, these results confirmed that applied K-oximes improved the oxidative status and further harmful systemic effects of rats after subacute administration.

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亚急性给药不同k -肟后大鼠脑组织对氧磷酶活性及促氧化-抗氧化平衡的测定

本研究旨在测定选择的k -肟亚急性治疗后Wistar大鼠脑组织中对氧磷酶活性和促氧化-抗氧化平衡。以0.1 LD50/kg的剂量肌注k -肟，每周2次，连续4周，末次给药后第7天，测定脑匀浆中对氧氧化酶活性（PON1）、促氧化-抗氧化平衡（PAB）、超氧阴离子自由基（O2•-）水平、亚硝酸盐（NO2−）浓度和游离蛋白巯基含量。几乎所有肟处理组的PON1和PAB活性均显著降低(p <；0.01和p <；分别为0.001)。奥比多肟-、K048-、K074-和k075处理组O2•-浓度显著升高(p <；0.001)，而阿索肟-、奥比肟-、K074和k075处理大鼠的NO2 -水平显著降低(p <；0.01, p <；分别为0.001)。各肟处理组的硫醇组含量均显著升高(p <；0.001)。继续我们之前发表的数据，这些结果证实，应用k -肟改善了亚急性给药后大鼠的氧化状态和进一步的有害全身效应。

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来源期刊

Chemico-Biological Interactions 生物-毒理学

CiteScore

7.70

自引率

3.90%

发文量

410

审稿时长

36 days

期刊介绍： Chemico-Biological Interactions publishes research reports and review articles that examine the molecular, cellular, and/or biochemical basis of toxicologically relevant outcomes. Special emphasis is placed on toxicological mechanisms associated with interactions between chemicals and biological systems. Outcomes may include all traditional endpoints caused by synthetic or naturally occurring chemicals, both in vivo and in vitro. Endpoints of interest include, but are not limited to carcinogenesis, mutagenesis, respiratory toxicology, neurotoxicology, reproductive and developmental toxicology, and immunotoxicology.