Whole genome sequence data of Bacillus anthracis strain 3B1 isolated from rice soil

IF 1 Q3 MULTIDISCIPLINARY SCIENCES

Data in Brief Pub Date : 2025-04-29 DOI:10.1016/j.dib.2025.111586

Rosamond Chan , Kah-Ooi Chua , Kelly Wan-Ee Teo , Dedat Prismantoro , Nurul Shamsinah Mohd Suhaimi , Abdullah Bilal Ozturk , Nia Rossiana , Febri Doni

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Abstract

Strain 3B1 was isolated from the soil of rice field cultivated under the system of rice intensification (SRI) in Sukabumi, West Java, Indonesia. The genome of strain 3B1 was sequenced using the MGI DNBSEQ platform, followed by bioinformatics processing, including genome assembly and gene annotation using SPAdes and Prokka, respectively. The assembled genome had a total length of 5,137,985 bp, distributed across 70 contigs, with 5,364 genes identified. Strain 3B1 shared the highest 16S rRNA gene sequence identity including Bacillus paranthracis, B. nitratireducens, B. cereus, B. paramycoides, B. tropicus, and B. anthracis, in the range of 99.86 to 99.93%. Both 16S rRNA gene and core genes-based phylogenetic analyses placed strain 3B1 in the same clade with B. anthracis strain Ames within the Bacillus genus. The phylogenetic placement was supported by the highest average nucleotide identity (ANI) value of 98.1% and digital DNA-DNA hybridization (dDDH) value of 82.7% shared between the genomes of B. anthracis strain Ames and strain 3B1, indicating that 3B1 is a strain of B. anthracis. Further gene annotation revealed that the genome of strain 3B1 lacked the genes encoding for virulence factors such as the pag, cya, and lef. Nonetheless, this data provides valuable insights into the genomic feature of strain 3B1, which can be bioprospected for various biotechnological applications.

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水稻土壤炭疽芽孢杆菌3B1株全基因组序列数据

菌株3B1是从印度尼西亚西爪哇苏卡布米水稻集约化栽培的水稻土壤中分离得到的。采用MGI DNBSEQ平台对菌株3B1进行基因组测序，然后分别使用SPAdes和Prokka进行生物信息学处理，包括基因组组装和基因注释。组装的基因组全长5137985 bp，分布在70个contigs中，鉴定出5364个基因。菌株3B1的16S rRNA基因序列同源性最高，在99.86 ~ 99.93%之间，分别为副芽孢杆菌、硝化还原芽孢杆菌、蜡样芽孢杆菌、副副芽孢杆菌、热带芽孢杆菌和炭疽芽孢杆菌。基于16S rRNA基因和核心基因的系统发育分析表明，菌株3B1与炭疽芽孢杆菌属菌株Ames属于同一进化支。菌株Ames与菌株3B1基因组的平均核苷酸同源性（ANI）最高，为98.1%，数字DNA-DNA杂交（dDDH）值最高，为82.7%，表明3B1为炭疽芽孢杆菌。进一步的基因注释显示，菌株3B1的基因组缺乏编码pag、cya和left等毒力因子的基因。尽管如此，这些数据为菌株3B1的基因组特征提供了有价值的见解，可以为各种生物技术应用进行生物勘探。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Data in Brief MULTIDISCIPLINARY SCIENCES-

CiteScore

3.10

自引率

0.00%

发文量

996

审稿时长

70 days

期刊介绍： Data in Brief provides a way for researchers to easily share and reuse each other''s datasets by publishing data articles that: -Thoroughly describe your data, facilitating reproducibility. -Make your data, which is often buried in supplementary material, easier to find. -Increase traffic towards associated research articles and data, leading to more citations. -Open up doors for new collaborations. Because you never know what data will be useful to someone else, Data in Brief welcomes submissions that describe data from all research areas.