Evaluation of stability, physicochemical properties, in vitro release behavior, and antioxidant activity of α-tocopherol encapsulated in ethyl(hydroxyethyl)cellulose (EHEC) films

IF 5.3 2区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Molecular Liquids Pub Date : 2025-05-02 DOI:10.1016/j.molliq.2025.127645

Greice M. Zickuhr , Leandro G. Nandi , Juliana P. Dreyer , Rafael F.N. Quadrado , André R. Fajardo , Larissa N. Carli , Ismael C. Bellettini

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Abstract

This study aimed to develop ethyl hydroxyethyl cellulose (EHEC) films with antioxidant properties by incorporating an oil-in-water emulsion composed of ethyl hydroxyethyl cellulose and dodecanoic acid (EHEC-C₁₂), loaded with α-tocopherol. The EHEC-C₁₂ derivative, with degrees of substitution (DS) ranging from 9.0 to 20.0 %, exhibited pronounced hydrophobicity and enhanced surface activity, indicating surfactant-like behavior due to the presence of alkyl groups covalently bonded to the polymer backbone. This amphiphilic nature proved essential for stabilizing α-tocopherol within the emulsion system. Emulsions containing 1–3 wt% α-tocopherol, as well as free α-tocopherol (2 wt%), were incorporated into the EHEC matrix to form films. Embedding α-tocopherol in the films significantly improved its stability under ambient conditions for up to 72 days and under UV radiation (λ = 254 nm) for 6 h, as well as increased its thermal degradation temperature (from 200 to 263 °C). The use of the EHEC-C₁₂ emulsion promoted a more uniform dispersion of α-tocopherol in the polymeric network, with minimal effects on the films’ barrier, thermal, and mechanical properties. Rapid release of α-tocopherol was observed in absolute ethanol, with approximately 50 % released within the first 15 min. Additionally, the incorporation of α-tocopherol enhanced the antioxidant activity of the films, which increased proportionally with its concentration.

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乙基（羟乙基）纤维素（EHEC）膜包封α-生育酚的稳定性、理化性质、体外释放行为及抗氧化活性评价

以乙基羟乙基纤维素（EHEC）和十二烷酸（EHEC- c12）为原料制备具有抗氧化性能的水包油乳液，并负载α-生育酚。EHEC-C12衍生物的取代度（DS）在9.0 ~ 20.0%之间，表现出明显的疏水性和增强的表面活性，表明由于共价键合在聚合物主链上的烷基的存在而表现出类似表面活性剂的行为。这种两亲性被证明是在乳液体系中稳定α-生育酚所必需的。将含有1-3 wt% α-生育酚和游离α-生育酚（2 wt%）的乳剂掺入EHEC基质中形成膜。α-生育酚包埋后，其在环境条件下的稳定性可达72天，在紫外线（λ = 254 nm）下的稳定性可达6 h，热降解温度从200℃提高到263℃。EHEC-C12乳液的使用促进了α-生育酚在聚合物网络中更均匀的分散，对膜的阻隔性、热学和力学性能的影响最小。α-生育酚在无水乙醇中快速释放，15 min内释放量约为50%。α-生育酚的加入增强了膜的抗氧化活性，且随其浓度的增加而成比例增加。

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来源期刊

Journal of Molecular Liquids 化学-物理：原子、分子和化学物理

CiteScore

10.30

自引率

16.70%

发文量

2597

审稿时长

78 days

期刊介绍： The journal includes papers in the following areas: – Simple organic liquids and mixtures – Ionic liquids – Surfactant solutions (including micelles and vesicles) and liquid interfaces – Colloidal solutions and nanoparticles – Thermotropic and lyotropic liquid crystals – Ferrofluids – Water, aqueous solutions and other hydrogen-bonded liquids – Lubricants, polymer solutions and melts – Molten metals and salts – Phase transitions and critical phenomena in liquids and confined fluids – Self assembly in complex liquids.– Biomolecules in solution The emphasis is on the molecular (or microscopic) understanding of particular liquids or liquid systems, especially concerning structure, dynamics and intermolecular forces. The experimental techniques used may include: – Conventional spectroscopy (mid-IR and far-IR, Raman, NMR, etc.) – Non-linear optics and time resolved spectroscopy (psec, fsec, asec, ISRS, etc.) – Light scattering (Rayleigh, Brillouin, PCS, etc.) – Dielectric relaxation – X-ray and neutron scattering and diffraction. Experimental studies, computer simulations (MD or MC) and analytical theory will be considered for publication; papers just reporting experimental results that do not contribute to the understanding of the fundamentals of molecular and ionic liquids will not be accepted. Only papers of a non-routine nature and advancing the field will be considered for publication.