Establishment of patient-derived 3D in vitro models of sarcomas: literature review and guidelines on behalf of the FORTRESS working group

IF 4.8 2区医学 Q1 Biochemistry, Genetics and Molecular Biology

Neoplasia Pub Date : 2025-05-03 DOI:10.1016/j.neo.2025.101171

Lore De Cock , Ieva Palubeckaitė , Francesca Bersani , Tobias Faehling , Sandro Pasquali , Sam Umbaugh , Michael Torsten Meister , Molly R. Danks , Piotr Manasterski , Richard Miallot , Manuela Krumbholz , Siyer Roohani , Dominique Heymann , Florencia Cidre-Aranaz , Agnieszka Wozniak , Patrick Schöffski , Judith V.M.G. Bovée , Alessandra Merlini , Sanne Venneker

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引用次数: 0

Abstract

Sarcomas are a large family of rare and heterogeneous mesenchymal tumors, which respond poorly to available systemic treatments. Translation of preclinical findings into clinical applications has been slow, limiting improvements in patients’ outcomes and ultimately highlighting the need for a better understanding of sarcoma biology to develop more effective, subtype-specific therapies. To this end, reliable preclinical models are crucial, but the development of 3D in vitro sarcoma models has been lagging behind that of epithelial cancers. This is primarily due to the rarity and heterogeneity of sarcomas, and lack of widespread knowledge regarding the optimal growth conditions of these in vitro models. In this review, we provide an overview of currently available sarcoma tumoroid models, together with guidelines and suggestions for model development and characterization, on behalf of the FORTRESS (Forum For Translational Research in Sarcomas) international research working group on 3D sarcoma models.

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建立患者来源的体外3D肉瘤模型：代表FORTRESS工作组进行文献综述和指南

肉瘤是一种罕见的异质性间充质肿瘤，对现有的全身治疗反应不佳。将临床前研究结果转化为临床应用一直很缓慢，限制了患者预后的改善，并最终强调需要更好地了解肉瘤生物学，以开发更有效的亚型特异性治疗方法。为此，可靠的临床前模型至关重要，但体外3D肉瘤模型的开发一直落后于上皮性癌症的开发。这主要是由于肉瘤的稀有性和异质性，以及缺乏关于这些体外模型的最佳生长条件的广泛知识。在这篇综述中，我们代表FORTRESS (Forum for Translational Research In sarcoma) 3D肉瘤模型国际研究工作组，概述了目前可用的肉瘤类肿瘤模型，以及模型开发和表征的指南和建议。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neoplasia 医学-肿瘤学

CiteScore

9.20

自引率

2.10%

发文量

审稿时长

26 days

期刊介绍： Neoplasia publishes the results of novel investigations in all areas of oncology research. The title Neoplasia was chosen to convey the journal’s breadth, which encompasses the traditional disciplines of cancer research as well as emerging fields and interdisciplinary investigations. Neoplasia is interested in studies describing new molecular and genetic findings relating to the neoplastic phenotype and in laboratory and clinical studies demonstrating creative applications of advances in the basic sciences to risk assessment, prognostic indications, detection, diagnosis, and treatment. In addition to regular Research Reports, Neoplasia also publishes Reviews and Meeting Reports. Neoplasia is committed to ensuring a thorough, fair, and rapid review and publication schedule to further its mission of serving both the scientific and clinical communities by disseminating important data and ideas in cancer research.