Effect of low intensity-magnetic field coupled with blue light on β-carotene synthesis in Blakeslea trispora

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing Pub Date : 2025-04-26 DOI:10.1016/j.fbp.2025.04.014

Dongxu Wang , Junwei Bao , Luqian Gong , Jing Bai , Yong Wang , Jinlong Liu

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Abstract

Blakeslea trispora is a promising microorganism used for the industrial production of β-carotene due to its rapid growth and high yield. However, the effects of magnetic fields and blue light on β-carotene synthesis in B. trispora remains have not been fully elucidated. For the first time, this paper investigated the effects of low-intensity magnetic field (LI-MF) and photomagnetic coupling on the synthesis of β-carotene by B. trispora. The results showed that the content of β-carotene increased from 3.77 mg/g to 7.57 mg/g under LI-MF. The production of β-carotene following the combination of LI-MF with blue light was lower compared to LI-MF treatment alone, yet higher than the control. It is hypothesized that the metabolic processes mediated by LI-MF and blue light interfere with each other. Transcriptome analysis indicated that LI-MF enhanced glucose metabolism, amino acid metabolism, and transporter pathways. Additionally, genes related to nucleobase-containing compound transport, and RNA localization were significantly affected by the co-treatment of LI-MF and blue light compared to LI-MF treatment. Overall, this study analyzed the enhancement of β-carotene production by LI-MF and the interaction mechanism between LI-MF and blue light in B. trispora at the molecular level, providing valuable insights for the application of LI-MF and blue light in B. trispora.

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弱磁场与蓝光耦合对三孢子黑藻中β-胡萝卜素合成的影响

三孢子黑霉因其生长快、产量高，是一种很有前途的β-胡萝卜素工业生产微生物。然而，磁场和蓝光对三孢子b残体中β-胡萝卜素合成的影响尚未完全阐明。本文首次研究了低强度磁场和光磁耦合对三孢霉合成β-胡萝卜素的影响。结果表明，在LI-MF作用下，β-胡萝卜素含量由3.77 mg/g增加到7.57 mg/g。与单独处理相比，LI-MF与蓝光联合处理后β-胡萝卜素的产量较低，但高于对照组。假设LI-MF和蓝光介导的代谢过程相互干扰。转录组分析表明，LI-MF增强了葡萄糖代谢、氨基酸代谢和转运蛋白途径。此外，与LI-MF处理相比，LI-MF和蓝光共处理显著影响了含核碱基化合物转运和RNA定位相关基因。综上所述，本研究在分子水平上分析了LI-MF对三孢子b β-胡萝卜素生成的促进作用以及LI-MF与蓝光的相互作用机制，为LI-MF和蓝光在三孢子b中的应用提供了有价值的见解。

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来源期刊

Food and Bioproducts Processing 工程技术-工程：化工

CiteScore

9.70

自引率

4.30%

发文量

115

审稿时长

24 days

期刊介绍： Official Journal of the European Federation of Chemical Engineering: Part C FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering. Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing. The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those: • Primarily concerned with food formulation • That use experimental design techniques to obtain response surfaces but gain little insight from them • That are empirical and ignore established mechanistic models, e.g., empirical drying curves • That are primarily concerned about sensory evaluation and colour • Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material, • Containing only chemical analyses of biological materials.