Transcriptomic analysis reveals the molecular mechanisms of heterosis in low-temperature tolerance in the hybrids of Argopecten scallops

IF 2.2 2区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-04-30 DOI:10.1016/j.cbd.2025.101526

Peican Zhu , Guizhen Wang , Yuan Liu , Lisen Wen , Qixiang Bo , Guilong Liu , Chunde Wang , Bo Liu

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Abstract

The F₁ hybrid of Argopecten irradians irradians (♀) × A. purpuratus (♂) exhibits significant heterosis in growth performance and mid-parent heterosis in low-temperature tolerance. This study presents a comparative transcriptomic analysis of A. irradians irradians (Ai), A. purpuratus (Ap), and the hybrid A. irradians irradians♀ × A. purpuratus♂ (Aip) to explore the mechanisms underlying low-temperature tolerance heterosis in Aip. A total of 33,376 differentially expressed genes (DEGs) were identified between F₁ hybrids and purebreds under cold stress. In Aip, 80.32 % of DEGs exhibited non-additive expression patterns, with over-dominant expression observed in 30.65 % of these genes. Pairwise comparisons among the transcriptomes of Ai, Ap, and Aip revealed 14,959 alternative splicing events, affecting 8169 genes. KEGG pathway analysis indicated substantial enrichment of overlapping genes from common DEGs and non-additively expressed genes (NAGs) in apoptosis, longevity regulation, ABC transporters, and spliceosome pathways. Furthermore, analysis of DEGs, DAGs (Differentially Alternative Splicing genes), and NAGs identified 6 genes undergoing alternative splicing. These pathways and genes may be crucial in Aip's response to low-temperature stress and offer insights for advancing scallop cross-breeding strategies.

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转录组学分析揭示了亚贝扇贝杂种耐低温优势的分子机制

结果表明：1代杂交后代的生长性能和耐低温性均具有显著的中亲本优势。本研究通过对a . irradians irradians （Ai）、a . purpuratus （Ap）和杂种a . irradians irradians♀× a . purpuratus♂（Aip）的转录组比较分析，探讨Aip耐低温杂种优势的机制。在低温胁迫下，F1杂交种与纯种共鉴定出33,376个差异表达基因（deg）。在Aip中，80.32%的deg表现为非加性表达模式，其中30.65%的deg表现为过显性表达。对Ai、Ap和Aip的转录组进行两两比较，发现14,959个可变剪接事件，影响8169个基因。KEGG通路分析表明，在细胞凋亡、寿命调节、ABC转运体和剪接体通路中，常见的deg和非加性表达基因（nag）的重叠基因大量富集。此外，通过对deg、DAGs （differential Alternative Splicing genes）和nag的分析，鉴定出6个进行选择性剪接的基因。这些途径和基因可能在Aip对低温胁迫的反应中起着至关重要的作用，并为推进扇贝的杂交育种策略提供了见解。

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来源期刊

Comparative Biochemistry and Physiology D-Genomics & Proteomics 生物-生化与分子生物学

CiteScore

5.10

自引率

3.30%

发文量

审稿时长

33 days

期刊介绍： Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology. Part D: Genomics and Proteomics (CBPD), focuses on “omics” approaches to physiology, including comparative and functional genomics, metagenomics, transcriptomics, proteomics, metabolomics, and lipidomics. Most studies employ “omics” and/or system biology to test specific hypotheses about molecular and biochemical mechanisms underlying physiological responses to the environment. We encourage papers that address fundamental questions in comparative physiology and biochemistry rather than studies with a focus that is purely technical, methodological or descriptive in nature.