Characterization and genomic analysis of Lactiplantibacillus plantarum LP8 as a probiotic candidate for medical applications

IF 1 Q3 MULTIDISCIPLINARY SCIENCES

Data in Brief Pub Date : 2025-04-11 DOI:10.1016/j.dib.2025.111555

Nirusna Jehma , Nattarika Chaichana , Jirasa Boonsan , Kamonnut Singkhamanan , Monwadee Wonglapsuwan , Rattanaruji Pomwised , Sarunyou Chusri , Komwit Surachat

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引用次数: 0

Abstract

This study presents the whole genome sequencing (WGS) and functional analysis of Lactiplantibacillus plantarum LP8, a promising probiotic strain, is presented in this research. The genome comprises a 3.23 Mbp circular chromosome and three plasmids including plasmid1_LP8 (58,764 bp), plasmid2_LP8 (45,003 bp), and plasmid3_LP8 (7985 bp). Functional annotation identified 3151 coding sequences (CDSs) mapped to 209 RAST subsystems, alongside biosynthesis gene clusters encoding Plantaricin J and other secondary metabolites such as RiPP-like peptides and terpenes. Safety analysis revealed no acquired antimicrobial resistance gene (AMR) or virulence genes, with only intrinsic resistance to certain antibiotics. In vitro analysis confirmed its sensitivity to antibiotics such as ampicillin, erythromycin, and tetracycline, and its hemolytic activity displayed an α-hemoly-sis pattern. These findings confirm L. plantarum LP8 as a safeand effective probiotic candidate with significant potential for antimicrobial and biotechnological applications.

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植物乳杆菌LP8作为医学应用的候选益生菌的特性和基因组分析

本研究对植物乳杆菌LP8 （Lactiplantibacillus plantarum LP8）进行了全基因组测序和功能分析。基因组包括一条3.23 Mbp的环状染色体和三个质粒，包括plasmid1_LP8 （58,764 bp）、plasmid2_LP8 （45,003 bp）和plasmid3_LP8 （7985 bp）。功能注释鉴定出3151个编码序列（CDSs），与209个RAST子系统相关联，以及编码Plantaricin J和其他次生代谢产物（如ripp样肽和萜烯）的生物合成基因簇。安全性分析显示无获得性耐药基因（AMR）或毒力基因，仅对某些抗生素具有内在耐药。体外分析证实其对氨苄西林、红霉素、四环素等抗生素敏感，其溶血活性呈α-溶血型。这些发现证实了植物乳杆菌LP8是一种安全有效的候选益生菌，具有重要的抗菌和生物技术应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Data in Brief MULTIDISCIPLINARY SCIENCES-

CiteScore

3.10

自引率

0.00%

发文量

996

审稿时长

70 days

期刊介绍： Data in Brief provides a way for researchers to easily share and reuse each other''s datasets by publishing data articles that: -Thoroughly describe your data, facilitating reproducibility. -Make your data, which is often buried in supplementary material, easier to find. -Increase traffic towards associated research articles and data, leading to more citations. -Open up doors for new collaborations. Because you never know what data will be useful to someone else, Data in Brief welcomes submissions that describe data from all research areas.