The role of IFT20 mediated by neuropeptide FILa1 in ciliary activity during larval settlement of Urechis unicinctus

IF 2.8 2区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Frontiers in Marine Science Pub Date : 2025-04-28 DOI:10.3389/fmars.2025.1575455

Long Zhang, Wenqing Zhang, Wenyuan Lai, Zhi Yang, Dawei Lin, Zhengrui Zhang, Zhifeng Zhang

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引用次数: 0

Abstract

BackgroundSettlement and metamorphosis are crucial developmental events in the life cycles of most marine benthic invertebrates. Neuropeptides play an important role in inducing larval settlement. However, studies on the ciliary genes and the few studies have examined the pathways regulated by neuropeptides during larval settlement.MethodsHere, we employed multiple molecular biology techniques to identify a ciliary gene, intraflagellar transport protein 20 (IFT20) which played a significant role in the larval settlement of Urechis unicinctus (Annelida, Echiura), and revealed the related gene pathway of IFT20 expression regulated by FILa1, a neuropeptide unique to U. unicinctus.ResultsThe IFT20 protein of U. unicinctus was highly conserved with that of other animals and localized at the ciliary base in the trochophore and the segmentation larvae of U. unicinctus. It was determined that the neuropeptide FILa1 inhibits IFT20 expression through the cAPM-PKA-CREB and Ca2+ pathways, which in turn triggers larval settlement. Knockdown the mRNA level of IFT20 resulted in a reduction in the number of vesicles at the ciliary base, the β-tubulin protein synthesis, the number and length of cilia, and the beat frequency of the circumoral cilia. Finally, the settlement rate of the larvae in the IFT20-dsRNA group increased by 3.64 times and 2.88 times compared to the control group at 48 and 72 h, respectively.ConclusionOur findings provide new insights into the function of IFT20 and the signaling pathways through which neuropeptides regulate ciliary gene expression during larval settlement.

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神经肽FILa1介导的IFT20在unicucus幼虫定居过程中纤毛活性中的作用

沉降和变态是大多数海洋底栖无脊椎动物生命周期中至关重要的发育事件。神经肽在诱导幼虫定居中起重要作用。然而，对纤毛基因的研究和少数研究对幼虫定居过程中神经肽调控的途径进行了研究。方法采用多种分子生物学技术，鉴定了在unicinctus（环节动物，Echiura）幼虫定居过程中起重要作用的纤毛基因——鞭毛内转运蛋白20 (IFT20)，并揭示了unicinctus特有的神经肽FILa1调控IFT20表达的相关基因通路。结果独角螨IFT20蛋白与其他动物的IFT20蛋白高度保守，定位于独角螨针蚴和分节幼虫的纤毛基部。我们确定神经肽FILa1通过cAPM-PKA-CREB和Ca2+途径抑制IFT20的表达，从而触发幼虫定居。敲低IFT20 mRNA水平导致纤毛基部囊泡数量减少，β-微管蛋白合成减少，纤毛数量和长度减少，环纤毛跳动频率减少。最后，在48 h和72 h时，IFT20-dsRNA组幼虫的沉降率分别比对照组提高了3.64倍和2.88倍。结论本研究为揭示IFT20的功能及幼虫定居过程中神经肽调控纤毛基因表达的信号通路提供了新的思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Marine Science Agricultural and Biological Sciences-Aquatic Science

CiteScore

5.10

自引率

16.20%

发文量

2443

审稿时长

14 weeks

期刊介绍： Frontiers in Marine Science publishes rigorously peer-reviewed research that advances our understanding of all aspects of the environment, biology, ecosystem functioning and human interactions with the oceans. Field Chief Editor Carlos M. Duarte at King Abdullah University of Science and Technology Thuwal is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, policy makers and the public worldwide. With the human population predicted to reach 9 billion people by 2050, it is clear that traditional land resources will not suffice to meet the demand for food or energy, required to support high-quality livelihoods. As a result, the oceans are emerging as a source of untapped assets, with new innovative industries, such as aquaculture, marine biotechnology, marine energy and deep-sea mining growing rapidly under a new era characterized by rapid growth of a blue, ocean-based economy. The sustainability of the blue economy is closely dependent on our knowledge about how to mitigate the impacts of the multiple pressures on the ocean ecosystem associated with the increased scale and diversification of industry operations in the ocean and global human pressures on the environment. Therefore, Frontiers in Marine Science particularly welcomes the communication of research outcomes addressing ocean-based solutions for the emerging challenges, including improved forecasting and observational capacities, understanding biodiversity and ecosystem problems, locally and globally, effective management strategies to maintain ocean health, and an improved capacity to sustainably derive resources from the oceans.