Morpho-phylogenetic and toxicological characterisation of Pseudo-nitzschia multiseries from the Sea of Marmara (Türkiye)

IF 5.5 1区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Harmful Algae Pub Date : 2025-04-21 DOI:10.1016/j.hal.2025.102867

Rabia Sezgin , Turgay Durmus , Muharrem Balci , Fuat Dursun , Neslihan Balkis-Ozdelice , Bernd Krock , Seyfettin Tas

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引用次数: 0

Abstract

Pseudo-nitzschia multiseries is a diatom known for producing domoic acid (DA), a neurotoxin that can cause Amnesic Shellfish Poisoning (ASP), which poses risks to both marine life and human health. This study reports the first detailed investigation of P. multiseries in the Sea of Marmara, focusing on its morphology, phylogeny, and toxin production. Morphological analysis using light and electron microscopy confirmed key diagnostic features consistent with P. multiseries. Phylogenetic analysis, focusing on ITS and LSU gene sequences, showed a close genetic relationship between the Turkish strain and other strains of P. multiseries. Domoic acid levels, quantified using LC-MS/MS during exponential and stationary growth phases, ranged between 2.46 and 3.24 pg cell⁻¹, with minor amounts of isoDA (A, D and E) also detected. These findings highlight the importance of monitoring P. multiseries in Turkish coastal waters due to its significant potential for DA production. This study provides valuable insights into the morphology, phylogeny, and toxin production of P. multiseries, as well as its potential implications for management of marine resources and public health protection. It offers a comprehensive examination of this potentially toxic diatom species in Turkish coastal waters.

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马尔马拉海伪尼茨氏菌多系的形态系统发育和毒理学特征

伪耐氏多系列硅藻是一种以产生软骨藻酸（DA）而闻名的硅藻，软骨藻酸是一种神经毒素，可导致健忘性贝类中毒（ASP），对海洋生物和人类健康都构成威胁。本研究报告了马尔马拉海多系列假单胞菌的首次详细调查，重点研究了其形态、系统发育和毒素产生。光镜和电镜形态学分析证实了与多系疟原虫一致的主要诊断特征。通过ITS和LSU基因序列的系统发育分析，发现土耳其菌株与其他菌株亲缘关系密切。在指数和平稳生长阶段，使用LC-MS/MS定量测定软骨藻酸水平，范围在2.46和3.24 pg cell - 1之间，也检测到少量的isoDA （A， D和E）。这些发现强调了监测土耳其沿海水域多系列P.的重要性，因为它具有巨大的DA生产潜力。本研究对多系假单胞菌的形态、系统发育和毒素产生及其对海洋资源管理和公众健康保护的潜在影响提供了有价值的见解。它提供了对土耳其沿海水域这种潜在有毒硅藻物种的全面检查。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Harmful Algae 生物-海洋与淡水生物学

CiteScore

12.50

自引率

15.20%

发文量

122

审稿时长

7.5 months

期刊介绍： This journal provides a forum to promote knowledge of harmful microalgae and macroalgae, including cyanobacteria, as well as monitoring, management and control of these organisms.