Identification of novel long non-coding RNA involved in Sertoli cell of non-obstructive azoospermia based on microarray and bioinformatics analysis

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2025-04-23 DOI:10.1016/j.ygeno.2025.111046

Danial Hashemi Karoii , Ali Shakeri Abroudi , Maryam Darvar , Melika Djamali , Hossein Azizi , Thomas Skutella

{"title":"Identification of novel long non-coding RNA involved in Sertoli cell of non-obstructive azoospermia based on microarray and bioinformatics analysis","authors":"Danial Hashemi Karoii , Ali Shakeri Abroudi , Maryam Darvar , Melika Djamali , Hossein Azizi , Thomas Skutella","doi":"10.1016/j.ygeno.2025.111046","DOIUrl":null,"url":null,"abstract":"<div><div>Non-obstructive azoospermia (NOA) is a severe form of male infertility, yet its underlying molecular mechanisms remain poorly understood. This study aimed to identify key regulatory non-coding RNAs (ncRNAs) and hub genes associated with NOA through an integrative bioinformatics approach. Using microarray analysis, we examined 4956 ncRNAs and identified 29 differentially expressed ncRNAs (14 upregulated, 15 downregulated) in NOA compared to healthy individuals. Co-expression analysis revealed significant interactions between lncRNAs, miRNAs, and mRNAs, predicting 31 target mRNAs within the regulatory network. Further, single-cell transcriptomic analysis identified four pivotal hub genes in NOA Sertoli cells: CLTC, XIAP, and DHFR (upregulated) and STMN1 (downregulated). Functional enrichment analysis highlighted critical pathways, including mitotic spindle organization and phosphatase activity, suggesting their involvement in NOA pathophysiology. Our findings provide novel insights into the molecular mechanisms underlying NOA and propose potential biomarkers for improved diagnosis and therapeutic strategies.</div></div>","PeriodicalId":12521,"journal":{"name":"Genomics","volume":"117 3","pages":"Article 111046"},"PeriodicalIF":3.4000,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genomics","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S088875432500062X","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Non-obstructive azoospermia (NOA) is a severe form of male infertility, yet its underlying molecular mechanisms remain poorly understood. This study aimed to identify key regulatory non-coding RNAs (ncRNAs) and hub genes associated with NOA through an integrative bioinformatics approach. Using microarray analysis, we examined 4956 ncRNAs and identified 29 differentially expressed ncRNAs (14 upregulated, 15 downregulated) in NOA compared to healthy individuals. Co-expression analysis revealed significant interactions between lncRNAs, miRNAs, and mRNAs, predicting 31 target mRNAs within the regulatory network. Further, single-cell transcriptomic analysis identified four pivotal hub genes in NOA Sertoli cells: CLTC, XIAP, and DHFR (upregulated) and STMN1 (downregulated). Functional enrichment analysis highlighted critical pathways, including mitotic spindle organization and phosphatase activity, suggesting their involvement in NOA pathophysiology. Our findings provide novel insights into the molecular mechanisms underlying NOA and propose potential biomarkers for improved diagnosis and therapeutic strategies.

查看原文本刊更多论文

基于微阵列和生物信息学分析的非阻塞性无精子症支持细胞相关长链非编码RNA的鉴定

非阻塞性无精子症（NOA）是男性不育症的一种严重形式，但其潜在的分子机制尚不清楚。本研究旨在通过综合生物信息学方法鉴定与NOA相关的关键调控非编码rna （ncRNAs）和枢纽基因。通过微阵列分析，研究人员检测了4956种ncrna，并鉴定出与健康个体相比，NOA中29种差异表达的ncrna（14种上调，15种下调）。共表达分析揭示了lncrna、mirna和mrna之间的显著相互作用，预测了调控网络中的31个靶mrna。此外，单细胞转录组学分析确定了NOA支持细胞中的四个关键枢纽基因：CLTC， XIAP和DHFR（上调）和STMN1（下调）。功能富集分析强调了关键途径，包括有丝分裂纺锤体组织和磷酸酶活性，表明它们参与了NOA的病理生理。我们的研究结果为NOA的分子机制提供了新的见解，并为改进诊断和治疗策略提供了潜在的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.