Green synthesis, characterization, structural, morphological, antibacterial, and cytotoxicity evaluation of zinc oxide nanoparticles using Fioria vitifolia extract

Abstract

The increasing prevalence of bacterial pathogens diseases and the rise in multidrug resistance highlights the urgent need for new drug delivery systems or novel drug molecules to enhance treatment options. Zinc oxide (ZnO) nanoparticles attracting attention due to their potential in biomedical applications, such as cancer therapy and diagnostics. ZnO is a versatile compound with excellent UV-blocking, anti-inflammatory, and wide-bandgap semiconductor properties. This study focuses on the green synthesis of ZnO nanoparticles using ‘Fioria vitifolia’ leaf extract, as a reducing agent with polyvinylpyrrolidone (PVP) aids in reducing particle size and preventing aggregation, enhancing nanoparticle stability. The ZnO nanoparticles were characterized using various techniques, including X-ray diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM), Energy-Dispersive X-ray Analysis (EDX), Transmission Electron Microscopy (TEM), Fourier-Transform Infrared Spectroscopy (FTIR), UV–Vis Diffuse Reflectance Spectroscopy (DRS), and Photoluminescence (PL). These analyses confirmed the successful formation of ZnO nanoparticles. The nanoparticles demonstrated strong antimicrobial activity, especially against ‘Enterobacter’, and exhibited significant cytotoxic effects on lung cancer cells (A549), but has low toxicity to standard cells (L929). The IC₅₀ values affirmed their potential as anticancer agents, suggesting their dual promise as antimicrobial and anticancer compounds. The enormous potential of biosynthesized ZnO nanoparticles as biological agents a sustainable substitute for chemically synthesized medications is highlighted in this study. The potential of the nanoparticles in a range of biomedical applications is highlighted by their ecologically friendly manufacturing process as well as their proven antibacterial and anticancer qualities.