Chemical stress assessment in large rivers using the average biomarker response approach with caged fish in situ: a case study on the Danube River in Novi Sad (Serbia)

IF 6 3区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES
Dina Tenji, Sonja Kaišarević, Bojana Mićić, Mirna Velki, Tobias Schulze, Martin Krauss, Muhammad Arslan Kamal Hashmi, Werner Brack, Ivana Teodorović
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引用次数: 0

Abstract

Background

Exposure assessment of aquatic biota to complex chemical mixtures from anthropogenic sources and evaluating potential impacts are crucial for mitigating toxic pressure and improving ecological status. The study focused on a section of the Danube River impacted by untreated municipal wastewater from the city of Novi Sad, Serbia, as well as partly treated industrial effluents and agricultural runoffs. This research aimed to implement average biomarker response (ABR) approach and use it as one of the multiple lines of evidence (LOE) to assess the ecological impact of pollution in complex scenarios, such as those in large rivers with multiple stressors.

Results

Cage-exposed specimens of native fish species, Cyprinus carpio, were analyzed for a wide range of biomarkers. Biomarker responses from caged fish were integrated with the biomarker responses of fish from wild populations of Abramis brama into an ABR value and interpreted using supporting evidence such as chemical analyses, effect-based methods and biomonitoring. Higher ABR values at pollution-impacted sites confirmed the presence of chronic toxic pressure caused by mixtures of chemicals at sublethal concentrations. Certain groups of biomarkers, such as those indicating neurotoxic effects, pointed to specific types of pollution confirmed by chemical analyses. Combined with other lines of evidence, ABR provided biologically plausible evidence of ecological impact of pollution at this hot spot and proved that pollution originating from the urban zone of the city of Novi Sad represents a significant pressure on the Danube ecosystem.

Conclusions

The integrated approach effectively assesses complex exposure scenarios in large rivers, evaluating pollution impact and underscoring the chronic toxic stress from anthropogenic sources. The assessment of biomarker responses in situ, whether based on caging experiments or sentinel biota, requires a comprehensive range of biomarkers and is overly resource-intensive for regular monitoring. Still, ecological relevance of the results justifies the efforts when the ecological impact of pollution needs to be assessed. ABR copes with high sample variability typical for in situ experiments, enables integration of inconsistent biomarker response patterns and facilitates the interpretation of the results and potentially wider acceptance of in situ tools in decision-making.

利用笼鱼的平均生物标志物反应法评估大河中的化学压力:以诺维萨德(塞尔维亚)多瑙河为例
水生生物群对复杂化学混合物的暴露评估和潜在影响评估对于减轻毒性压力和改善生态状况至关重要。这项研究的重点是多瑙河的一段河段,该段河段受到塞尔维亚诺维萨德市未经处理的城市废水以及部分处理的工业废水和农业径流的影响。本研究旨在实施平均生物标志物反应(ABR)方法,并将其作为多证据线(LOE)方法之一,以评估复杂情景下污染的生态影响,例如具有多种压力源的大河。结果对本地鱼类鲤科鱼(Cyprinus carpio)暴露的标本进行了广泛的生物标志物分析。将笼养鱼的生物标志物响应与野生布拉马鱼种群的生物标志物响应整合到ABR值中,并使用化学分析、基于效应的方法和生物监测等支持证据进行解释。在受污染影响的地点,较高的ABR值证实存在由亚致死浓度的化学品混合物引起的慢性毒性压力。某些生物标记,如那些表明神经毒性作用的生物标记,指出了化学分析证实的特定类型的污染。结合其他证据,ABR提供了污染在这一热点地区的生态影响的生物学上合理的证据,并证明来自诺维萨德市市区的污染对多瑙河生态系统构成了重大压力。结论该综合方法可有效评估大型河流的复杂暴露情景,评估污染影响并强调人为源的慢性毒性应激。就地评估生物标志物的反应,无论是基于笼式实验还是哨兵生物群,都需要全面的生物标志物,而且对于定期监测来说,资源过于密集。尽管如此,当需要评估污染的生态影响时,结果的生态相关性证明了努力的合理性。ABR处理了原位实验中典型的高样本可变性,能够整合不一致的生物标志物反应模式,促进了结果的解释,并可能在决策中更广泛地接受原位工具。
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来源期刊
Environmental Sciences Europe
Environmental Sciences Europe Environmental Science-Pollution
CiteScore
11.20
自引率
1.70%
发文量
110
审稿时长
13 weeks
期刊介绍: ESEU is an international journal, focusing primarily on Europe, with a broad scope covering all aspects of environmental sciences, including the main topic regulation. ESEU will discuss the entanglement between environmental sciences and regulation because, in recent years, there have been misunderstandings and even disagreement between stakeholders in these two areas. ESEU will help to improve the comprehension of issues between environmental sciences and regulation. ESEU will be an outlet from the German-speaking (DACH) countries to Europe and an inlet from Europe to the DACH countries regarding environmental sciences and regulation. Moreover, ESEU will facilitate the exchange of ideas and interaction between Europe and the DACH countries regarding environmental regulatory issues. Although Europe is at the center of ESEU, the journal will not exclude the rest of the world, because regulatory issues pertaining to environmental sciences can be fully seen only from a global perspective.
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