Isolation and Identification of Plant Growth-Promoting Endophytes Bacteria Highly Effective in Suppressing Damping-off in Sugar Beet

IF 1.8 3区农林科学 Q2 AGRONOMY

Sugar Tech Pub Date : 2025-02-12 DOI:10.1007/s12355-025-01547-9

Bingchen Zou, Tai Li, Yonglong Liu, Zeyuan Cui, Lihua Yu, Gui Geng, Jiahui Liu, Yao Xu, Yuguang Wang

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引用次数: 0

Abstract

Sugar beet is an important sugar producing cash crop in the world with high economic value, but the crop faces a great economic loss every year due to damping-off. The concept of interactions produced between plant endophytic bacteria and host plants as an important biological control are gaining importance. In the present study, a strain of sugar beet standoff antagonist was obtained through plate standoff isolation and purification. Morphological observation, comparison of physiological and biochemical characteristics, and 16S rRNA gene sequences analysis were conducted for the classification and identification of the bacterial strain. The strain sb-13 was identified as Bacillus altitudinis. Through potting experiments, the growth of sugar beet inoculated with and without sb-13 was compared to analyze the effect of the endophytic bacteria on the growth of sugar beet, and it was found that the bacterium has the properties of promoting plant growth such as solubilizing phosphorus and dissolving potassium and enhancing the ability of soil enzyme activity. The experimental results showed that the growth of sugar beet inoculated with sb-13 was significantly better than that of the control group, and its physiological and biochemical indicators were significantly improved. In the anti-blight experiment, it was found that the bacterium antagonist had the ability to possess cellulose degradation and pectinase, and the incidence rate of sugar beet damping-off inoculated with sb-13 was significantly lower than that of the control group. This study provides a new idea for the biological control of sugar beet damping-off.

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甜菜高产促生长内生菌的分离与鉴定

甜菜是世界上重要的制糖经济作物，具有很高的经济价值，但每年因受潮而遭受巨大的经济损失。植物内生细菌与寄主植物之间的相互作用作为一种重要的生物防治手段越来越受到重视。本研究通过平板对峙分离纯化获得了一株甜菜对峙拮抗剂。通过形态学观察、生理生化特征比较、16S rRNA基因序列分析对菌株进行分类鉴定。菌株sb-13鉴定为高原芽孢杆菌。通过盆栽试验，比较了接种和未接种sb-13对甜菜生长的影响，分析了内生细菌对甜菜生长的影响，发现该细菌具有溶磷、溶钾、提高土壤酶活性等促进植物生长的特性。实验结果表明，接种sb-13后甜菜的生长情况明显好于对照组，其生理生化指标均有显著改善。在抗枯萎病试验中，发现拮抗菌剂具有纤维素降解和果胶酶的能力，接种sb-13后的甜菜阻尼率显著低于对照组。本研究为甜菜萎蔫病的生物防治提供了新的思路。

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来源期刊

Sugar Tech AGRONOMY-

CiteScore

3.90

自引率

21.10%

发文量

145

期刊介绍： The journal Sugar Tech is planned with every aim and objectives to provide a high-profile and updated research publications, comments and reviews on the most innovative, original and rigorous development in agriculture technologies for better crop improvement and production of sugar crops (sugarcane, sugar beet, sweet sorghum, Stevia, palm sugar, etc), sugar processing, bioethanol production, bioenergy, value addition and by-products. Inter-disciplinary studies of fundamental problems on the subjects are also given high priority. Thus, in addition to its full length and short papers on original research, the journal also covers regular feature articles, reviews, comments, scientific correspondence, etc.