Yellowfin tuna (Thunnus albacares) intestinal tissues: a sustainable marine resource for the recovery of alkaline proteases with potential application in detergent formulations and non-aqueous catalysis media

IF 2.7 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES
Gissel Daniela Rios-Herrera, Idalia Osuna-Ruiz, Emmanuel Martínez-Montaño, Víctor Alfonso Rodríguez-Tirado, Mario Alberto Miguel-Velarde, María Fernanda Trujillo-Lizárraga, Andrés Nahúm Torres-Robles, Israel Benítez-García, Jesús Aarón Salazar-Leyva
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引用次数: 0

Abstract

Yellowfin tuna (Thunnus albacares) is one of the most captured species for industrial processing and their viscera usually are considered a waste that represents a low-cost source of proteolytic enzymes. Thus, the stability of crude alkaline proteases extracted from yellowfin tuna intestinal tissues, to several denaturant compounds was evaluated. The proteolytic activity was retained more than 60% at NaCl concentrations of 5 to 15% (w/v) and when the protease extract was incubated with oxidizing (H2O2) and surfactant (SDS and Tween 80) agents, the residual enzyme activity was maintained similar to control with no agent (P > 0.05). Also, yellowfin tuna crude alkaline proteases, exhibited around 70% of residual enzyme activity when were incubated in solid and liquid detergents, suggesting its compatibility with the ingredients of commercial formulations of laundry detergents. The crude alkaline proteases were stable after incubation in ethanol, methanol and 2-propanol for 1 h and even for 2 days. The obtained results, suggest that yellowfin tuna viscera can be exploited as a sustainable marine resource for the recovery of enzymes with potential industrial application.

黄鳍金枪鱼(Thunnus albacares)肠道组织:回收碱性蛋白酶的可持续海洋资源,在洗涤剂配方和非水催化介质中具有潜在的应用前景
黄鳍金枪鱼(Thunnus albacares)是工业加工中捕获最多的物种之一,它们的内脏通常被认为是一种废物,代表了一种低成本的蛋白水解酶来源。因此,从黄鳍金枪鱼肠组织中提取的粗碱性蛋白酶对几种变性化合物的稳定性进行了评价。在NaCl浓度为5 ~ 15% (w/v)的条件下,蛋白酶提取物的酶解活性保持在60%以上;在氧化剂(H2O2)和表面活性剂(SDS和Tween 80)作用下,蛋白酶提取物的酶解活性保持在与不加任何活性剂的对照组相似的水平(P > 0.05)。此外,黄鳍金枪鱼粗碱性蛋白酶在固体和液体洗涤剂中均表现出70%左右的残留酶活性,表明其与商业洗衣粉配方成分的相容性。粗碱性蛋白酶在乙醇、甲醇和2-丙醇中孵育1 h,甚至2 d后均保持稳定。研究结果表明,黄鳍金枪鱼内脏可以作为一种可持续的海洋资源,用于酶的回收,具有潜在的工业应用价值。
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来源期刊
CiteScore
5.30
自引率
16.10%
发文量
205
审稿时长
4.8 months
期刊介绍: The Journal of Material Cycles and Waste Management has a twofold focus: research in technical, political, and environmental problems of material cycles and waste management; and information that contributes to the development of an interdisciplinary science of material cycles and waste management. Its aim is to develop solutions and prescriptions for material cycles. The journal publishes original articles, reviews, and invited papers from a wide range of disciplines related to material cycles and waste management. The journal is published in cooperation with the Japan Society of Material Cycles and Waste Management (JSMCWM) and the Korea Society of Waste Management (KSWM).
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