A Survey on Sugarcane Mosaic Symptoms Leads to the First Report of Sugarcane Chlorotic Streak Virus in Cameroon

IF 1.8 3区农林科学 Q2 AGRONOMY

Sugar Tech Pub Date : 2025-01-09 DOI:10.1007/s12355-024-01527-5

Jean H. Daugrois, Benoît Ahondopké, Serge Galzi, Charlotte Julian, Denis Filloux, Philippe C. Rott, Philippe Roumagnac

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引用次数: 0

Abstract

Twenty-five leaf samples collected from 17 sugarcane varieties from Cameroon were sent to Cirad in France for virus diagnosis. Four sugarcane leaf samples from four varieties showing mosaic-like symptoms tested negative by RT-PCR for potyviruses and a poacevirus causing sugarcane mosaic and sugarcane streak mosaic in sugarcane, respectively. Virus sequences sharing 98–99% nucleotide identity with sugarcane chlorotic streak virus (SCSV) (Renamed Mastrevirus saccharumpallidi; ICTV 2024) isolates from Nigeria were found in these four samples by virion-associated nucleic acid metagenomics. Five complete genome sequences of SCSV from Cameroon were obtained by rolling circle amplification, cloning, and sequencing. These five genome sequences had 97.8–98.7% identity with the genome sequences of 12 SCSV isolates from Nigeria. The 17 full genome sequences of SCSV isolates from Cameroon and Nigeria shared 75–76% nucleotide identity with members of the closest Mastrevirus species (Eragrostis streak virus renamed Mastrevirus eragrostis and Urochloa streak virus renamed Mastrevirus urochloae by the International Committee on Taxonomy of Viruses). To our knowledge, this is the first report of SCSV in Cameroon.

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对甘蔗花叶症状的调查导致喀麦隆甘蔗褪绿条纹病毒的首次报道

从喀麦隆17个甘蔗品种采集的25个叶片样本被送往法国Cirad进行病毒诊断。RT-PCR检测结果显示，4个甘蔗品种的4个甘蔗叶片分别对引起甘蔗花叶病和甘蔗条纹花叶病的多型病毒和一种多型病毒呈阴性反应。与甘蔗褪绿条纹病毒（SCSV）(重命名为saccharumpallidi Mastrevirus；病毒相关核酸宏基因组学分析在这4份样本中发现尼日利亚产ICTV 2024)分离株。通过滚圈扩增、克隆和测序，获得了5个喀麦隆SCSV全基因组序列。这5个基因组序列与尼日利亚12株SCSV分离株基因组序列的同源性为97.8 ~ 98.7%。来自喀麦隆和尼日利亚的SCSV分离株的17个全基因组序列与最接近的Mastrevirus物种（国际病毒分类委员会将Eragrostis条纹病毒更名为Mastrevirus Eragrostis， Urochloa条纹病毒更名为Mastrevirus urochloae）的成员具有75-76%的核苷酸同源性。据我们所知，这是喀麦隆首例SCSV报告。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sugar Tech AGRONOMY-

CiteScore

3.90

自引率

21.10%

发文量

145

期刊介绍： The journal Sugar Tech is planned with every aim and objectives to provide a high-profile and updated research publications, comments and reviews on the most innovative, original and rigorous development in agriculture technologies for better crop improvement and production of sugar crops (sugarcane, sugar beet, sweet sorghum, Stevia, palm sugar, etc), sugar processing, bioethanol production, bioenergy, value addition and by-products. Inter-disciplinary studies of fundamental problems on the subjects are also given high priority. Thus, in addition to its full length and short papers on original research, the journal also covers regular feature articles, reviews, comments, scientific correspondence, etc.