Systems Metabolic Engineering of Clostridium tyrobutyricum for 1,3‐Propanediol Production From Crude Glycerol

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology and Bioengineering Pub Date : 2025-04-21 DOI:10.1002/bit.29010

Yongzhang Mo, Xiaolong Guo, Yang Lan, Jufang Wang, Hongxin Fu

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引用次数: 0

Abstract

Clostridium tyrobutyricum has emerged as a non‐pathogenic microbial cell factory capable of anaerobic production of various value‐added products, such as butyrate, butanol, and butyl butyrate. This study reports the first systematic engineering of C. tyrobutyricum for the heterologous production of 1,3‐propanediol (1,3‐PDO) from industrial by‐product crude glycerol. Initially, the glycerol reductive pathway for 1,3‐PDO production was constructed, and the unique glycerol oxidation pathway in C. tyrobutyricum was elucidated. Subsequently, the glycerol metabolism and 1,3‐PDO synthesis pathways were enhanced. Furthermore, the intracellular reducing power supply and the fermentation process were optimized to improve 1,3‐PDO production. Consequently, 54.06 g/L 1,3‐PDO with a yield of 0.64 mol/mol and a productivity of 1.13 g/L·h was obtained using crude glycerol and fish meal. The strategies described herein could facilitate the engineering of C. tyrobutyricum as a robust host for synthesizing valuable chemicals.

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酪氨酸丁酸梭菌从粗甘油生产1,3 -丙二醇的系统代谢工程

酪酸丁酸梭菌已经成为一种非致病微生物细胞工厂，能够厌氧生产各种增值产品，如丁酸盐、丁醇和丁酸丁酯。本研究报道了C. tyrobutyricum从工业副产物粗甘油中异源生产1,3‐丙二醇（1,3‐PDO）的第一个系统工程。首先，构建了1,3 - PDO生成的甘油还原途径，并阐明了C. tyrobutyricum独特的甘油氧化途径。随后，甘油代谢和1,3‐PDO合成途径被增强。此外，优化了胞内还原电源和发酵工艺，以提高1,3‐PDO的产量。结果表明，使用粗甘油和鱼粉可得到54.06 g/L 1,3‐PDO，产率为0.64 mol/mol，产率为1.13 g/L·h。本文所述的策略可以促进C. tyrobutyricum作为合成有价值化学品的健壮宿主的工程。

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来源期刊

Biotechnology and Bioengineering 工程技术-生物工程与应用微生物

CiteScore

7.90

自引率

5.30%

发文量

280

审稿时长

2.1 months

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