Correction to “Sequential Assembly of Cell-Laden Hydrogel Constructs to Engineer Vascular-Like Microchannels”

Abstract

Y. Du, M. Ghodousi, H. Qi, N. Haas, W. Xiao, and A. Khademhosseini, “Sequential Assembly of Cell-Laden Hydrogel Constructs to Engineer Vascular-Like Microchannels,” Biotechnology and Bioengineering 108, no. 7 (2011): 1693–1703, 10.1002/bit.23102.

This correction is published due to concerns raised by a third party regarding high similarity between the cell-laden microgels between Figure 4B,D.

The authors explained that the images in Figure 4B,D were from the same sample that was sequentially exposed to different conditions (first exposed to PI and subsequently put in oil) and analyzed. Thus, the microgels appeared repositioned between the imaging sessions. Due to the elapsed time since publication, the original image files were not available, therefore the authors have repeated the experiment in which the gels were not sequentially analyzed to verify the conclusion that “each assembly phase is conducive to cell viability.”

The new data confirmed the same trends as observed before, therefore the experimental results and the corresponding conclusions of the paper remain unaffected.

The corrected Figure 4 is below:

Figure 4

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Viability tests for the cell-laden tubular hydrogels as a function of the steps in the assembly process. The sequential assembly procedure was shown to be cell-friendly as demonstrated by viability tests for: (A) 3T3 fibroblast-laden microgels; (B) microgels washed three times with photoinitiator (PI) (1% in DPBS); (C) microgels exposed to oil; (D) microgels exposed to both PI and oil; (E) microgel assembly after 2 days in culture medium; (F) quantification of samples (n = 3) after each fabrication step showed that the assembly process did not result in a significant loss of cell viability. Scale bar: 500 µm.