Inducing elevated glucose levels in vitro: A model to simulate prediabetes (preDBT) states in primary cultures

IF 8.8 2区医学 Q1 IMMUNOLOGY

Brain, Behavior, and Immunity Pub Date : 2025-04-14 DOI:10.1016/j.bbi.2025.04.019

Canal Maria Pilar , Acutain Maria Florencia , Nini Karen Agustina , Munner Mariana , Caruso Ornella , De Tomas-Liorio Anna , Badollati Adelina , Baez María Verónica

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引用次数: 0

Abstract

There is increasing evidence suggesting a relationship between prediabetes (preDBT, the early stage of Type 2 Diabetes or DBT2) and neurodegenerative disorders (NDDs) such as Alzheimer’s disease. The preDBT stage, characterized by impaired fasting glucose (IFG), may represent an early risk factor for cognitive decline and the onset of NDDs. However, the underlying mechanisms connecting preDBT to cognitive impairment and neurodegeneration remain poorly understood. This study aims to explore the effects of IFG on central nervous system (CNS) cells by developing an in vitro model of preDBT using sera from individuals with IFG. Our results demonstrate that exposure of astrocyte-neuron mixed cultures to IFG sera induced hyperglycemia, increased oxidative levels and astrogliosis that would lead to cognitive impairment observed in the analyzed cohort, as evidenced by a battery of cognitive tests. These findings suggest that the early stages of preDBT may trigger changes in CNS cells that correlate with cognitive decline. The study underscores the importance of early diagnosis and intervention in preDBT to prevent progression to DBT2 and associated NDDs.

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体外诱导葡萄糖水平升高：在原代培养中模拟前驱糖尿病（preDBT）状态的模型

越来越多的证据表明，前驱糖尿病（preDBT， 2型糖尿病或DBT2的早期阶段）与神经退行性疾病（ndd）如阿尔茨海默病之间存在关系。以空腹血糖（IFG）受损为特征的前dbt阶段可能是认知能力下降和ndd发病的早期危险因素。然而，将preDBT与认知障碍和神经退行性变联系起来的潜在机制仍然知之甚少。本研究旨在通过使用IFG患者的血清建立preDBT体外模型，探讨IFG对中枢神经系统（CNS）细胞的影响。我们的研究结果表明，星形细胞-神经元混合培养物暴露于IFG血清中会导致高血糖、氧化水平升高和星形胶质细胞增生，这在分析的队列中观察到，这将导致认知障碍，正如一系列认知测试所证明的那样。这些发现表明，前驱dbt的早期阶段可能引发与认知能力下降相关的中枢神经系统细胞的变化。该研究强调了早期诊断和干预dbt前期的重要性，以防止发展为DBT2和相关的ndd。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Brain, Behavior, and Immunity 医学-免疫学

CiteScore

29.60

自引率

2.00%

发文量

290

审稿时长

28 days

期刊介绍： Established in 1987, Brain, Behavior, and Immunity proudly serves as the official journal of the Psychoneuroimmunology Research Society (PNIRS). This pioneering journal is dedicated to publishing peer-reviewed basic, experimental, and clinical studies that explore the intricate interactions among behavioral, neural, endocrine, and immune systems in both humans and animals. As an international and interdisciplinary platform, Brain, Behavior, and Immunity focuses on original research spanning neuroscience, immunology, integrative physiology, behavioral biology, psychiatry, psychology, and clinical medicine. The journal is inclusive of research conducted at various levels, including molecular, cellular, social, and whole organism perspectives. With a commitment to efficiency, the journal facilitates online submission and review, ensuring timely publication of experimental results. Manuscripts typically undergo peer review and are returned to authors within 30 days of submission. It's worth noting that Brain, Behavior, and Immunity, published eight times a year, does not impose submission fees or page charges, fostering an open and accessible platform for scientific discourse.