The role of testosterone in modulating positive and negative empathy in social interactions

Abstract

Empathy encompasses both negative (e.g., distress) and positive (e.g., shared joy) dimensions, yet the effects of testosterone on positive empathy and its modulation of intrinsic neural dynamics remain underexplored. This double-blind, placebo-controlled study examined how testosterone influences neural sensitivity to empathy within social inclusion and exclusion contexts, as well as its impact on resting-state EEG microstates—millisecond-scale transient patterns of brain activity. Healthy male participants received either testosterone or placebo before completing resting-state EEG recordings and an empathy task featuring social scenarios. While self-reported empathy ratings remained unchanged, testosterone amplified neurophysiological responses: it enhanced anterior N2 amplitude (250–310 ms), associated with negative empathy toward social exclusion, and increased posterior α-event-related desynchronization (8.28–10 Hz; 1226–1901 ms), linked to positive empathy during social inclusion. These findings suggest that testosterone enhances neural responsiveness to both threatening and affiliative social cues, reinforcing its role in adaptive social vigilance. Resting-state EEG microstate analysis further revealed that testosterone prolonged the temporal dominance of microstate E—a centro-parietal activity pattern associated with interoceptive awareness and emotional processing. Notably, these microstate E changes predicted increased emotional empathy across both positive and negative contexts. Together, our findings suggest that testosterone indirectly enhances empathy-related responsiveness by amplifying baseline interoceptive sensitivity to socially salient stimuli. These dual effects—enhanced intrinsic interoceptive processing and heightened neural reactivity to social cues—position testosterone as a key neuromodulator of context-adaptive social perception.