Enzyme Mimic Behavior of Platinum(II) Complex with Tridentate Ligand—Fe3O4—MWCNT Nanocomposite for the Electrochemical Detection of H2O2 and NADH

IF 2.7 4区化学 Q3 CHEMISTRY, PHYSICAL

Electrocatalysis Pub Date : 2025-01-10 DOI:10.1007/s12678-025-00927-9

İzzet Koçak, Fatih Pekdemir

{"title":"Enzyme Mimic Behavior of Platinum(II) Complex with Tridentate Ligand—Fe3O4—MWCNT Nanocomposite for the Electrochemical Detection of H2O2 and NADH","authors":"İzzet Koçak, Fatih Pekdemir","doi":"10.1007/s12678-025-00927-9","DOIUrl":null,"url":null,"abstract":"<div><p>A platinum-based complex molecule, [PtLCl]Cl (Pt-L), featuring a tridentate ligand (<i>L</i> = 2,6-bis(benzimidazol-2-yl)-4-hydroxypyridine) was synthesized. Subsequently, Pt-L was successfully bonded to amine-terminated Fe<sub>3</sub>O<sub>4</sub>NP, and MWCNTs were incorporated into the modified Fe<sub>3</sub>O<sub>4</sub> surface. The resulting electrode was shown to possess outstanding electrocatalytic activity for detecting H<sub>2</sub>O<sub>2</sub> and NADH, characterized by enhanced cathodic or anodic peak responses and favorable shifts in the reduction or oxidation peak potentials for both analytes. The sensing platform demonstrated excellent electrochemical performance in non-enzymatic measurements of H<sub>2</sub>O<sub>2</sub> and NADH, achieving notably low detection limits of 0.017 µmol L<sup>−1</sup> for H<sub>2</sub>O<sub>2</sub> and 0.113 µmol L<sup>−1</sup> for NADH. These findings were acquired within the concentration range of 10 to 500 µmol L<sup>−1</sup>, indicating the linear portion of the calibration graphs obtained in the concentration ranges of 10 to 5000 µmol L<sup>−1</sup> for H₂O₂ and 10 to 25,000 µmol L<sup>−1</sup> for NADH, which exhibited exponential behavior for both analytes. The developed sensor displayed high responsiveness, long-lasting stability, and requisite interference, rendering it suitable for routine detection of H<sub>2</sub>O<sub>2</sub> and NADH in biological specimens.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":535,"journal":{"name":"Electrocatalysis","volume":"16 3","pages":"391 - 403"},"PeriodicalIF":2.7000,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Electrocatalysis","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s12678-025-00927-9","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}

引用次数: 0

Abstract

A platinum-based complex molecule, [PtLCl]Cl (Pt-L), featuring a tridentate ligand (L = 2,6-bis(benzimidazol-2-yl)-4-hydroxypyridine) was synthesized. Subsequently, Pt-L was successfully bonded to amine-terminated Fe₃O₄NP, and MWCNTs were incorporated into the modified Fe₃O₄ surface. The resulting electrode was shown to possess outstanding electrocatalytic activity for detecting H₂O₂ and NADH, characterized by enhanced cathodic or anodic peak responses and favorable shifts in the reduction or oxidation peak potentials for both analytes. The sensing platform demonstrated excellent electrochemical performance in non-enzymatic measurements of H₂O₂ and NADH, achieving notably low detection limits of 0.017 µmol L⁻¹ for H₂O₂ and 0.113 µmol L⁻¹ for NADH. These findings were acquired within the concentration range of 10 to 500 µmol L⁻¹, indicating the linear portion of the calibration graphs obtained in the concentration ranges of 10 to 5000 µmol L⁻¹ for H₂O₂ and 10 to 25,000 µmol L⁻¹ for NADH, which exhibited exponential behavior for both analytes. The developed sensor displayed high responsiveness, long-lasting stability, and requisite interference, rendering it suitable for routine detection of H₂O₂ and NADH in biological specimens.

Graphical Abstract

查看原文本刊更多论文

铂（II）配合物与三齿配体- fe3o4 - mwcnt纳米复合材料的酶模拟行为用于电化学检测H2O2和NADH

合成了具有三齿配体（L = 2,6-双（苯并咪唑-2-基）-4-羟基吡啶）的铂基配合物[PtLCl]Cl （Pt-L）。随后，Pt-L成功与胺端Fe3O4NP结合，MWCNTs被掺入修饰后的Fe3O4表面。结果表明，该电极在检测H2O2和NADH方面具有出色的电催化活性，其特征是增强的阴极或阳极峰响应以及两种分析物的还原或氧化峰电位的有利变化。该传感平台在H2O2和NADH的非酶测中表现出优异的电化学性能，H2O2和NADH的检出限分别为0.017µmol L−1和0.113µmol L−1。这些结果是在10到500µmol L−1的浓度范围内获得的，表明在10到5000µmol L−1的H₂O₂和10到25000µmol L−1的NADH浓度范围内获得的校准图的线性部分，这两种分析物都表现出指数行为。该传感器具有响应性高、稳定性好、抗干扰能力强等特点，适用于生物标本中H2O2和NADH的常规检测。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Electrocatalysis CHEMISTRY, PHYSICAL-ELECTROCHEMISTRY

CiteScore

4.80

自引率

6.50%

发文量

审稿时长

>12 weeks

期刊介绍： Electrocatalysis is cross-disciplinary in nature, and attracts the interest of chemists, physicists, biochemists, surface and materials scientists, and engineers. Electrocatalysis provides the unique international forum solely dedicated to the exchange of novel ideas in electrocatalysis for academic, government, and industrial researchers. Quick publication of new results, concepts, and inventions made involving Electrocatalysis stimulates scientific discoveries and breakthroughs, promotes the scientific and engineering concepts that are critical to the development of novel electrochemical technologies. Electrocatalysis publishes original submissions in the form of letters, research papers, review articles, book reviews, and educational papers. Letters are preliminary reports that communicate new and important findings. Regular research papers are complete reports of new results, and their analysis and discussion. Review articles critically and constructively examine development in areas of electrocatalysis that are of broad interest and importance. Educational papers discuss important concepts whose understanding is vital to advances in theoretical and experimental aspects of electrochemical reactions.