Genome Mining Reveals a Carboxyl O-Methyltransferase Gatekeeping the Biosynthetic Divergence of Tryptophan Dimers

IF 4.9 1区化学 Q1 CHEMISTRY, ORGANIC

Organic Letters Pub Date : 2025-04-14 DOI:10.1021/acs.orglett.5c01012

Liang Ma, Zhiwen Liu, Wenjun Zhang, Yiguang Zhu, Liping Zhang, Weiliang Xiong, Xiao-Nian Li, Xinpeng Tian, Changsheng Zhang

引用次数: 0

Abstract

Bacterial tryptophan dimers comprise a large family of natural products with promising biological activities. Herein, we report the identification of a methyltransferase, SpmM1, which acts as a gatekeeping enzyme to govern the divergent biosynthesis of tryptophan dimers spiroindimicins (SPMs) and indimicins (IDMs) in Streptomyces marincola SCSIO 03032. Bioinformatics, genetic, and biochemical studies revealed that SpmM1-catalyzed carboxyl O-methylation enabled the common precursor of SPMs and IDMs to flux into the spiro-forming pathway to generate SPMs.

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基因组挖掘揭示了一个羧基o -甲基转移酶控制着色氨酸二聚体的生物合成分化

细菌色氨酸二聚体是一类具有良好生物活性的天然产物。本文中，我们报道了一种甲基转移酶SpmM1的鉴定，该酶作为一种守门酶，控制着海洋链霉菌SCSIO 03032中色氨酸二聚体旋红霉素（SPMs）和旋红霉素（IDMs）的不同生物合成。生物信息学、遗传学和生化研究表明，spmm1催化的羧基o-甲基化使SPMs和IDMs的共同前体进入螺旋形成途径，从而产生SPMs。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Organic Letters 化学-有机化学

CiteScore

9.30

自引率

11.50%

发文量

1607

审稿时长

1.5 months

期刊介绍： Organic Letters invites original reports of fundamental research in all branches of the theory and practice of organic, physical organic, organometallic,medicinal, and bioorganic chemistry. Organic Letters provides rapid disclosure of the key elements of significant studies that are of interest to a large portion of the organic community. In selecting manuscripts for publication, the Editors place emphasis on the originality, quality and wide interest of the work. Authors should provide enough background information to place the new disclosure in context and to justify the rapid publication format. Back-to-back Letters will be considered. Full details should be reserved for an Article, which should appear in due course.