Stimulation of bronchoalveolar lavage (BAL) and blood lymphocytes by Kveim antigen, tuberculin and concanavalin A in sarcoidosis

British journal of diseases of the chest Pub Date : 1988-01-01 DOI:10.1016/0007-0971(88)90093-9

M. Lindahl , O. Andersson , E. Ripe , G. Holm

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引用次数: 6

Abstract

BAL and blood mononuclear cells and their reactivity to Kveim antigen, tuberculin and concanavalin A (Con A) were studied in nine patients with different clinical stages of sarcoidosis. After separation by plastic adherence, non-adherent cells (mainly lymphocytes) were admixed with 10% autologous adherent cells (monocytes/macrophages). After 3 and 6 days' culture with Kveim antigen (1, 10, 100 μg/ml), PPD tuberculin (2.5 μg/ml) and Con A (10, 20, 40 μg/ml) stimulation was measured as incorporation of ¹⁴C-thymidine into DNA.

Except for occasional reactions the study did not show any unitary significant increase in lymphocyte response to the different concentrations of Kveim antigen in either BAL or blood. For Con A there was a weaker response by BAL-mononuclear cells with no difference between 3 and 6 days, compared with blood where there was an early peak. The lymphocyte reaction to PPD was weak with no difference between blood and BAL.

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结节病中Kveim抗原、结核菌素和豆蛋白A对支气管肺泡灌洗(BAL)和血液淋巴细胞的刺激作用

对9例不同临床分期结节病患者的BAL、血单个核细胞及其对Kveim抗原、结核菌素和豆蛋白A (cona)的反应性进行了研究。塑料贴壁分离后，将非贴壁细胞(主要是淋巴细胞)与10%的自体贴壁细胞(单核/巨噬细胞)混合。在Kveim抗原(1、10、100 μg/ml)、PPD结核菌素(2.5 μg/ml)和Con A(10、20、40 μg/ml)刺激下培养3、6天后，检测14c -胸腺嘧啶与DNA的结合情况。除了偶尔的反应外，研究并未显示淋巴细胞对BAL或血液中不同浓度的Kveim抗原的反应有任何单一的显著增加。对于Con A，与血液相比，在3天和6天内，bal -单核细胞的反应较弱，而血液则有一个早期的高峰。淋巴细胞对PPD的反应较弱，血液和BAL之间没有差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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British journal of diseases of the chest

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