Critical analysis of descriptive microRNA data in the translational research on cardioprotection and cardiac repair: lost in the complexity of bioinformatics

IF 7.5 1区 医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS
Mariann Gyöngyösi, Julia Guthrie, Ena Hasimbegovic, Emilie Han, Martin Riesenhuber, Kevin Hamzaraj, Jutta Bergler-Klein, Denise Traxler, Maximilian Y. Emmert, Matthias Hackl, Sophia Derdak, Dominika Lukovic
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引用次数: 0

Abstract

The unsuccessful translation of cardiac regeneration and cardioprotection from animal experiments to clinical applications in humans has raised the question of whether microRNA bioinformatics can narrow the gap between animal and human research outputs. We reviewed the literature for the period between 2000 and 2024 and found 178 microRNAs involved in cardioprotection and cardiac regeneration. On analyzing the orthologs and annotations, as well as downstream regulation, we observed species-specific differences in the diverse regulation of the microRNAs and related genes and transcriptomes, the influence of the experimental setting on the microRNA-guided biological responses, and database-specific bioinformatics results. We concluded that, in addition to reducing the number of in vivo experiments, following the 3R animal experiment rules, the bioinformatics approach allows the prediction of several currently unknown interactions between pathways, coding and non-coding genes, proteins, and downstream regulatory elements. However, a comprehensive analysis of the miRNA-mRNA-protein networks needs a profound bioinformatics and mathematical education and training to appropriately design an experimental study, select the right bioinformatics tool with programming language skills and understand and display the bioinformatics output of the results to translate the research data into clinical practice. In addition, using in-silico approaches, a risk of deviating from the in vivo processes exists, with adverse consequences on the translational research.

心脏保护和心脏修复转化研究中描述性microRNA数据的批判性分析:迷失在生物信息学的复杂性中
心脏再生和心脏保护从动物实验到人类临床应用的不成功转化引发了microRNA生物信息学是否可以缩小动物和人类研究成果之间差距的问题。我们回顾了2000年至2024年间的文献,发现了178种参与心脏保护和心脏再生的microrna。通过对同源物和注释以及下游调控的分析,我们观察到microrna及其相关基因和转录组的多种调控存在物种特异性差异,实验环境对microrna引导的生物反应的影响,以及数据库特异性生物信息学结果。我们的结论是,除了减少体内实验的数量外,遵循3R动物实验规则,生物信息学方法允许预测一些目前未知的途径,编码和非编码基因,蛋白质和下游调控元件之间的相互作用。然而,全面分析mirna - mrna -蛋白网络需要深入的生物信息学和数学教育和训练,以适当地设计实验研究,选择正确的生物信息学工具,并具有编程语言技能,理解和显示结果的生物信息学输出,将研究数据转化为临床实践。此外,使用硅片方法,存在偏离体内过程的风险,对转化研究产生不利影响。
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来源期刊
Basic Research in Cardiology
Basic Research in Cardiology 医学-心血管系统
CiteScore
16.30
自引率
5.30%
发文量
54
审稿时长
6-12 weeks
期刊介绍: Basic Research in Cardiology is an international journal for cardiovascular research. It provides a forum for original and review articles related to experimental cardiology that meet its stringent scientific standards. Basic Research in Cardiology regularly receives articles from the fields of - Molecular and Cellular Biology - Biochemistry - Biophysics - Pharmacology - Physiology and Pathology - Clinical Cardiology
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