Evaluation of the effectiveness of human breast milk exosomes in experimental testicular torsion-detorsion injury model.

Cirugia y cirujanos Pub Date : 2025-01-01 DOI:10.24875/CIRU.23000462

Ünal T Öztürk, Hatice S Y Cömert, Gül Şalcı, Ahmet Alver, Sevdegül A Mungan, Neslihan Sağlam, Şeniz Erdem, Serdar Karakullukçu, Mustafa İmamoğlu, Haluk Sarıhan

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Abstract

Objective: Our aim was to examine the effectiveness of human breast milk exosomes in treating testicular torsion.

Methods: Rats were divided randomly into three equal groups. Group 1 was our control group. A torsion/detorsion model was created for the rats in Group 2. After the torsion/detorsion model, 1 mg/kg intraperitoneally human breast milk exosomes were given 30 min before detorsion in Group 3. All testicles were removed at the 6^th h of the experiment, for biochemical and histopathological examination.

Results: There was a statistically significant decrease in Group 3 MDA values compared to Group 2. When Group 3 and Group 2 SOD and CAT values were compared, it was seen that these values were higher in Group 3. Histopathologically a statistically significant difference was found in Group 3 compared to Group 1 and Group 2 in terms of ischemia/reperfusion injury.

Conclusion: We have shown that human breast milk exosomes in testicular torsion do not completely prevent reperfusion injury but significantly preserve testicular tissue.

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人母乳外泌体在实验性睾丸扭转-扭转损伤模型中的有效性评价。

目的：探讨人母乳外泌体治疗睾丸扭转的疗效。方法：将大鼠随机分为3组。第一组是我们的对照组。第二组大鼠建立扭转/变形模型。扭转/扭转模型建立后，第三组在扭转前30 min腹腔注射人乳外泌体1 mg/kg。在实验第6 h切除所有睾丸，进行生化和组织病理学检查。结果：3组大鼠MDA值较2组降低，差异有统计学意义。比较3组和2组的SOD和CAT值，可以看出3组的SOD和CAT值较高。3组缺血再灌注损伤与1、2组比较，病理组织学差异有统计学意义。结论：人母乳外泌体在睾丸扭转中不能完全防止再灌注损伤，但能显著保护睾丸组织。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Cirugia y cirujanos

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