Production of Isoprimeverose from Xyloglucan Using Aspergillus oryzae.

Abstract

Isoprimeverose [α-D-xylopyranosyl-(1→6)-D-glucose] is produced from xyloglucan using the cooperative action of glycoside hydrolases including isoprimeverose-producing oligoxyloglucan hydrolase and β-galactosidase in Aspergillus oryzae. This study investigated A. oryzae strains and culture conditions suitable for isoprimeverose production from xyloglucan. Each strain of A. oryzae had a different ability to degrade xyloglucans. When an A. oryzae strain with high xyloglucan-degradation activity was cultured in a medium containing partially degraded xyloglucan as the carbon source, the production of glycoside hydrolases that degrade xyloglucan into isoprimeverose was highly induced. Our procedure efficiently produced isoprimeverose from xyloglucan without any genetically modified microorganisms or purification of enzymes.