MILLET BASED NUTRITIONAL SUPPLEMENT FOR DIARRHEAL EPISODE IN PROTEIN ENERGY MALNOURISHED CONDITION: AN EXPERIMENTAL APPROACH IN CASTOR OIL MODEL.

Q2 Medicine
Arquivos de Gastroenterologia Pub Date : 2025-04-04 eCollection Date: 2025-01-01 DOI:10.1590/S0004-2803.24612024-098
Sachin V Tembhurne, Mansi S Jagdale, Payal Kate, Ziyaurrahman Ar
{"title":"MILLET BASED NUTRITIONAL SUPPLEMENT FOR DIARRHEAL EPISODE IN PROTEIN ENERGY MALNOURISHED CONDITION: AN EXPERIMENTAL APPROACH IN CASTOR OIL MODEL.","authors":"Sachin V Tembhurne, Mansi S Jagdale, Payal Kate, Ziyaurrahman Ar","doi":"10.1590/S0004-2803.24612024-098","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Diarrhea is a gastrointestinal transit disorder and mostly seen in malnourished children's as per WHO report. Malnourished individuals are found to be associated with compromised immunity and lack of nutrients, which makes person susceptible to diarrhoea.</p><p><strong>Objective: </strong>For maintaining the gut health adequate and balance nutrition is essential. In this study, both fermented and non-fermented nutritional supplement was formulated and evaluated against castor-oil induced diarrhoea.</p><p><strong>Methods: </strong>Two groups of rats initially fed a 2% protein-deficient diet for ten weeks. After this period, one group received a diet enriched with nutritional components blended with fermented bovine colostrum, while the other group received a diet with non-fermented nutritional components. At the end of 20th week, castor oil was given to all animals except the control group to induce diarrhoea. Subsequently, these rats were subjected to various assessments including time of onset of the first diarrheal stool (min), faecal weight, faecal score, number of wet stools, intestinal fluid accumulation and Histopathological examination. DPPH radical-scavenging activity of nutritional blend was also determined.</p><p><strong>Results: </strong>The undernourished rats fed with non-fermented and fermented diet showed delayed the onset of diarrhea and reduction of weight stool, the decrease in the frequency and severity of defecation as well as significantly protected against the intestinal fluid accumulation as compare to negative control groups. The results showed that both the fermented and non-fermented blended composition exhibited antioxidant activity. The intestine of undernourished rats fed with fermented nutritional diet showed the absence of infiltration and improved villi structure.</p><p><strong>Conclusion: </strong>The study presents promising evidence of the potential benefits of the formulated nutritional compositions in alleviating the castor oil-induced diarrhea in undernourished wistar rats. The antioxidant activity, anti-diarrheal effects and improvements in gut histology suggest that, the nutritional compositions could be explored further as natural interventions for gastrointestinal health.</p><p><strong>Background: </strong>• Diarrhea is common in malnourished children due to compromised immunity and lack of nutrients.</p><p><strong>Background: </strong>• Rats were fed a protein-deficient diet (2%) for 10 weeks. One group received fermented bovine colostrum, while the other received non-fermented nutritional components.</p><p><strong>Background: </strong>• Diarrhea was induced in the animals, and they were evaluated for the onset of diarrhea, fecal weight, frequency, intestinal fluid accumulation, and histology.</p><p><strong>Background: </strong>• Both supplements delayed the onset of diarrhea, reduced its severity, and exhibited antioxidant activity.</p><p><strong>Background: </strong>• The nutritional compositions have potential benefits as natural interventions for intestinal health.</p>","PeriodicalId":35671,"journal":{"name":"Arquivos de Gastroenterologia","volume":"62 ","pages":"e24098"},"PeriodicalIF":0.0000,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12043190/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Arquivos de Gastroenterologia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1590/S0004-2803.24612024-098","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Diarrhea is a gastrointestinal transit disorder and mostly seen in malnourished children's as per WHO report. Malnourished individuals are found to be associated with compromised immunity and lack of nutrients, which makes person susceptible to diarrhoea.

Objective: For maintaining the gut health adequate and balance nutrition is essential. In this study, both fermented and non-fermented nutritional supplement was formulated and evaluated against castor-oil induced diarrhoea.

Methods: Two groups of rats initially fed a 2% protein-deficient diet for ten weeks. After this period, one group received a diet enriched with nutritional components blended with fermented bovine colostrum, while the other group received a diet with non-fermented nutritional components. At the end of 20th week, castor oil was given to all animals except the control group to induce diarrhoea. Subsequently, these rats were subjected to various assessments including time of onset of the first diarrheal stool (min), faecal weight, faecal score, number of wet stools, intestinal fluid accumulation and Histopathological examination. DPPH radical-scavenging activity of nutritional blend was also determined.

Results: The undernourished rats fed with non-fermented and fermented diet showed delayed the onset of diarrhea and reduction of weight stool, the decrease in the frequency and severity of defecation as well as significantly protected against the intestinal fluid accumulation as compare to negative control groups. The results showed that both the fermented and non-fermented blended composition exhibited antioxidant activity. The intestine of undernourished rats fed with fermented nutritional diet showed the absence of infiltration and improved villi structure.

Conclusion: The study presents promising evidence of the potential benefits of the formulated nutritional compositions in alleviating the castor oil-induced diarrhea in undernourished wistar rats. The antioxidant activity, anti-diarrheal effects and improvements in gut histology suggest that, the nutritional compositions could be explored further as natural interventions for gastrointestinal health.

Background: • Diarrhea is common in malnourished children due to compromised immunity and lack of nutrients.

Background: • Rats were fed a protein-deficient diet (2%) for 10 weeks. One group received fermented bovine colostrum, while the other received non-fermented nutritional components.

Background: • Diarrhea was induced in the animals, and they were evaluated for the onset of diarrhea, fecal weight, frequency, intestinal fluid accumulation, and histology.

Background: • Both supplements delayed the onset of diarrhea, reduced its severity, and exhibited antioxidant activity.

Background: • The nutritional compositions have potential benefits as natural interventions for intestinal health.

谷子营养补充剂治疗蛋白质能量营养不良腹泻:蓖麻油模型的实验方法。
背景:根据世界卫生组织的报告,腹泻是一种胃肠道传递障碍,主要见于营养不良儿童。发现营养不良的人与免疫力低下和缺乏营养有关,这使人容易腹泻。目的:为了维持肠道健康,充足和平衡的营养是必不可少的。在本研究中,配制了发酵和非发酵营养补充剂,并对蓖麻油引起的腹泻进行了评估。方法:两组大鼠先饲喂2%蛋白缺乏饲料10周。在这段时间后,一组饲喂混合了发酵牛初乳的营养成分丰富的日粮,而另一组饲喂不含发酵营养成分的日粮。第20周末,除对照组外,其余动物均给予蓖麻油诱导腹泻。随后,对这些大鼠进行各种评估,包括首次腹泻时间(分钟)、粪便重量、粪便评分、湿便数量、肠液积聚和组织病理学检查。测定了营养混合物对DPPH自由基的清除活性。结果:与阴性对照组相比,非发酵组和发酵组营养不良大鼠腹泻发作时间延迟,大便重量减轻,排便次数和严重程度降低,肠道积液明显减少。结果表明,发酵和未发酵的混合组合物均具有抗氧化活性。营养不良大鼠饲喂发酵营养日粮后,肠道无浸润,绒毛结构改善。结论:本研究提供了有希望的证据,表明所配制的营养成分在缓解营养不良的wistar大鼠蓖麻油引起的腹泻方面具有潜在的益处。这些营养成分的抗氧化活性、抗腹泻作用以及对肠道组织的改善表明,这些营养成分可以作为胃肠道健康的自然干预措施进一步探索。背景:•腹泻是常见的营养不良儿童由于免疫力低下和缺乏营养。•给大鼠喂食蛋白质缺乏饮食(2%)10周。一组饲喂发酵牛初乳,另一组饲喂非发酵营养成分。背景:•在动物中诱导腹泻,并评估它们的腹泻发作,粪便重量,频率,肠液积聚和组织学。背景:•两种补充剂均可延缓腹泻的发生,降低其严重程度,并表现出抗氧化活性。背景:•作为肠道健康的自然干预措施,营养成分具有潜在的益处。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Arquivos de Gastroenterologia
Arquivos de Gastroenterologia Medicine-Gastroenterology
CiteScore
2.00
自引率
0.00%
发文量
109
审稿时长
9 weeks
期刊介绍: The journal Arquivos de Gastroenterologia (Archives of Gastroenterology), a quarterly journal, is the Official Publication of the Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia IBEPEGE (Brazilian Institute for Studies and Research in Gastroenterology), Colégio Brasileiro de Cirurgia Digestiva - CBCD (Brazilian College of Digestive Surgery) and of the Sociedade Brasileira de Motilidade Digestiva - SBMD (Brazilian Digestive Motility Society). It is dedicated to the publishing of scientific papers by national and foreign researchers who are in agreement with the aim of the journal as well as with its editorial policies.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信