Vitamin D supplementation as a prophylactic therapy in the management of pre-eclampsia: Focus on VEGF, Ki67, oxidative stress markers in correlation to placental ultra structure

IF 5.2 2区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

Life sciences Pub Date : 2025-04-05 DOI:10.1016/j.lfs.2025.123605

Hanan Eissa , Eman Mohamed Abdelsalam , Somaia A. Mokbel , Nada H. Elhadedy , Rania M. Khalil , Amany AbdElfattah Mohamed AbdElfattah , Dalia M. Abdel Ghaffar , Eman Mohamad El Nashar , Alshehri Hanan Hassan , Norah Saeed Al-Zahrani , Rashid A. Aldahhan , Neimat Abd Elhakam Yassin

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引用次数: 0

Abstract

Background

Pre-eclampsia (PE) is a progressive hypertension condition that manifests in the second or third trimester of pregnancy and causes significant proteinuria. A lack of vitamin D (Vit. D) is linked to different pregnancy problems, including impaired placental development. Vitamin D has been shown to enhance fetal growth and lower the incidence of PE.

Aim of the work

To better understand the pathophysiological mechanisms behind the PE disease and the therapeutic approaches used to manage it, this study examines the role of Vit. D in placental ischemia and its regulatory effects in Nitro L-arginine Methyl Ester (L-NAME) animal model of PE.

Methods

Fifty female rats in the estrus stage were mated with 30 male rats. Thirty female rats were pregnant and divided into three equal groups: control, Preeclampsia group (PE); using L-NAME for induction of PE, and Vit. D group from 7th day then induction by L-NAME at 10th day till end of pregnancy. Mean arterial Bp, proteinuria, oxidative stress markers, histological structure and immunohistochemical expression of Ki67 and VEGF, Morphometric study, and transmission electron microscopy(TEM) were assessed. The results of the current study suggested that, Vit. D supplementation could lower blood pressure, reduce oxidative stress, and restore angiogenic balance through vascular endothelial growth factor (VEGF) and Ki67.

Conclusion

For the first time, we conclude that vitamin D supplementation may not only have direct effects on blood pressure regulation and angiogenic hemostasis but also recover placental function, actually contributing to the prevention or management of PE.

Abstract Image

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补充维生素D作为预防子痫前期的治疗：关注与胎盘超结构相关的VEGF、Ki67、氧化应激标志物

背景：先兆子痫（PE）是一种进行性高血压疾病，表现在妊娠中期或晚期，并引起显著的蛋白尿。缺乏维生素D。D)与不同的妊娠问题有关，包括胎盘发育受损。维生素D已被证明可以促进胎儿生长并降低PE的发生率。工作目的：为了更好地了解PE疾病背后的病理生理机制和治疗方法，本研究探讨了Vit的作用。D对胎盘缺血的影响及其在硝基精氨酸甲酯（L-NAME） PE动物模型中的调节作用。方法：50只处于发情期的雌性大鼠与30只雄性大鼠交配。将30只怀孕的雌性大鼠分为三组：对照组，子痫前期组（PE）；用L-NAME诱导PE、Vit。D组从第7天开始，第10天进行L-NAME诱导，直至妊娠结束。评估平均动脉血压、蛋白尿、氧化应激标志物、Ki67和VEGF的组织结构和免疫组织化学表达、形态计量学研究和透射电子显微镜（TEM）。目前的研究结果表明，Vit。补充维生素D可以通过血管内皮生长因子（VEGF）和Ki67降低血压，减少氧化应激，恢复血管生成平衡。结论：我们首次得出补充维生素D可能不仅对血压调节和血管生成止血有直接作用，而且可以恢复胎盘功能，实际上有助于预防或治疗PE。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Life sciences 医学-药学

CiteScore

12.20

自引率

1.60%

发文量

841

审稿时长

6 months

期刊介绍： Life Sciences is an international journal publishing articles that emphasize the molecular, cellular, and functional basis of therapy. The journal emphasizes the understanding of mechanism that is relevant to all aspects of human disease and translation to patients. All articles are rigorously reviewed. The Journal favors publication of full-length papers where modern scientific technologies are used to explain molecular, cellular and physiological mechanisms. Articles that merely report observations are rarely accepted. Recommendations from the Declaration of Helsinki or NIH guidelines for care and use of laboratory animals must be adhered to. Articles should be written at a level accessible to readers who are non-specialists in the topic of the article themselves, but who are interested in the research. The Journal welcomes reviews on topics of wide interest to investigators in the life sciences. We particularly encourage submission of brief, focused reviews containing high-quality artwork and require the use of mechanistic summary diagrams.