Roser Salvia, Laura G Rico, Teresa Morán, Michael W Olszowy, Michael D Ward, Jordi Petriz
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引用次数: 0
Abstract
Cellular cytotoxicity is an important mechanism of the immune system to clear infections and eliminate tumor cells. Its two main mediators are cytotoxic T lymphocytes and natural killer (NK) cells. In lung cancer, intratumoral NK cells show reduced cytolytic potential and one third of patients do not express HLA-I proteins, which activate NK cells in a process termed "absent self-recognition." In this work, we investigate NK cytotoxicity as a potential oncological biomarker that informs patient status and predicts response to treatment. We describe a simple and rapid test to analyze NK cytotoxicity without the need for large volumes of blood, requiring short processing time and reduced use of both reagents and blood samples, using the IncuCyte live imaging technique.
期刊介绍：
Cytometry Part A, the journal of quantitative single-cell analysis, features original research reports and reviews of innovative scientific studies employing quantitative single-cell measurement, separation, manipulation, and modeling techniques, as well as original articles on mechanisms of molecular and cellular functions obtained by cytometry techniques.
The journal welcomes submissions from multiple research fields that fully embrace the study of the cytome:
Biomedical Instrumentation Engineering
Biophotonics
Bioinformatics
Cell Biology
Computational Biology
Data Science
Immunology
Parasitology
Microbiology
Neuroscience
Cancer
Stem Cells
Tissue Regeneration.
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