β-cyclodextrin saves lysozyme from the clutches of 1-alkylsulfonates

IF 5.3 2区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Molecular Liquids Pub Date : 2025-04-05 DOI:10.1016/j.molliq.2025.127543

Ola Grabowska , Małgorzata M. Kogut-Günthel , Sergey A. Samsonov , Dariusz Wyrzykowski , Joanna Makowska

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引用次数: 0

Abstract

Non-covalent interactions between proteins and small molecules are one of the factors, along with pH and temperature, that have a significant impact on protein function, stability and biological activity. Reversible protein folding induced by molecular crowding can be controlled by introducing ligands that compete with the protein for small molecules. One example is lysozyme (Lys), which unfolds in the presence of ligands that contain hydrophobic groups in their structure, such as 1-alkylsulfonates (KXS). In this paper, a series of experimental methods, namely circular dichroism spectroscopy, isothermal titration calorimetry, conductometric titration and differential scanning calorimetry, supported by in silico analysis, have been applied to characterise the process of reversibility changes of lysozyme structure in the presence of 1-alkylsulfonates with different hydrophobic chain lengths and β-cyclodextrin (β-CD) used as a strong competitive KXS-binding ligand. It has been shown that the observed structural changes can be reversed by introducing β-CD into the system, which, due to its higher affinity for KXS in comparison to lysozyme, effectively binds small ligands and so allows the protein to refold.

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β-环糊精将溶菌酶从1-烷基磺酸盐的魔爪中拯救出来

蛋白质与小分子之间的非共价相互作用是与pH和温度一起对蛋白质功能、稳定性和生物活性产生重大影响的因素之一。由分子拥挤引起的可逆蛋白质折叠可以通过引入与蛋白质竞争小分子的配体来控制。溶菌酶（Lys）就是一个例子，它在结构中含有疏水性基团的配体（如1-烷基磺酸盐（KXS））的存在下展开。本文采用圆二色光谱法、等温滴定量热法、电导滴定法和差示扫描量热法等一系列实验方法，在硅分析的支持下，表征了不同疏水链长的1-烷基磺酸盐和作为强竞争kxs结合配体的β-环糊氨酸（β-CD）存在下溶菌酶结构的可逆性变化过程。研究表明，通过将β-CD引入体系可以逆转所观察到的结构变化，由于β-CD与溶菌酶相比对KXS具有更高的亲和力，因此可以有效地结合小配体，从而使蛋白质重新折叠。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Molecular Liquids 化学-物理：原子、分子和化学物理

CiteScore

10.30

自引率

16.70%

发文量

2597

审稿时长

78 days

期刊介绍： The journal includes papers in the following areas: – Simple organic liquids and mixtures – Ionic liquids – Surfactant solutions (including micelles and vesicles) and liquid interfaces – Colloidal solutions and nanoparticles – Thermotropic and lyotropic liquid crystals – Ferrofluids – Water, aqueous solutions and other hydrogen-bonded liquids – Lubricants, polymer solutions and melts – Molten metals and salts – Phase transitions and critical phenomena in liquids and confined fluids – Self assembly in complex liquids.– Biomolecules in solution The emphasis is on the molecular (or microscopic) understanding of particular liquids or liquid systems, especially concerning structure, dynamics and intermolecular forces. The experimental techniques used may include: – Conventional spectroscopy (mid-IR and far-IR, Raman, NMR, etc.) – Non-linear optics and time resolved spectroscopy (psec, fsec, asec, ISRS, etc.) – Light scattering (Rayleigh, Brillouin, PCS, etc.) – Dielectric relaxation – X-ray and neutron scattering and diffraction. Experimental studies, computer simulations (MD or MC) and analytical theory will be considered for publication; papers just reporting experimental results that do not contribute to the understanding of the fundamentals of molecular and ionic liquids will not be accepted. Only papers of a non-routine nature and advancing the field will be considered for publication.