A novel combination mode for inhalant allergen screening based on 8-well microplates by LiCA

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of immunological methods Pub Date : 2025-04-04 DOI:10.1016/j.jim.2025.113856

Yixian Li , Xiaoming Cui , Xiaohui Yang , Qinqin Liu , Yuanmin Sun , Xue Li , Huiqiang Li , Yang Yu

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引用次数: 0

Abstract

Objective

Currently, allergen detection is primarily classified into two types: single detection and multiple combined detection. The challenges include large volume of serum required, high testing costs, and fixed combinations of test items. A cost-effective method that uses a small amount of serum and allows for the flexible combination of allergen screening is necessary. Light-initiated Chemiluminescence Assay (LiCA) requires no washing, only need little serum, and supports flexible combination detection of allergens.

Methods

Based on the advantages of LiCA technology, which does not require washing, the ‘4 + 3X + 1’ allergen detection mode was established in this study. The antigens are coated on chemibeads, which are selectively added into the detection wells to achieve flexible combination, supporting the simultaneous detection of eight allergens.

Results

This study introduces a new allergen screening model termed “4 + 3X + 1”, where “1” represents tIgE, “4” comprises four commonly encountered indoor inhalable allergens, and “3X” encompasses a variety of frequent inhalable allergens. Allergens from “3X” could be selected based on regional characteristics and patient-specific conditions. We tested the intermediate precision(3.59 %–9.71 %) and repeatability(2.81 %–9.31 %) of the four allergens in “4-fixed”, and LoB(0.035–0.066 kU_A/L), LoD(0.092–0.156 kU_A/L), and LoQ(0.135–0.199 kU_A/L) all showed good results. We compared the consistency of LiCA and ImmunoCAP/Dot-ELISA, each of which was greater than 0.8828. In addition, two allergens were selected from “4-fixed” and “3X-optional “respectively to detect the sensitivity(80 %–97.72 %) and specificity(93.75 %–100 %).

Conclusions

The proposed combined test model offers a new, economical, and rapid option for allergen screening that requires low blood volumes and can be customized for individual patients.

查看原文本刊更多论文

基于 8 孔微孔板的新型 LiCA 吸入性过敏原筛选组合模式。

目的：目前过敏原检测主要分为单一检测和多种联合检测两种。挑战包括需要大量血清，检测成本高，以及检测项目的固定组合。需要一种使用少量血清并允许灵活结合过敏原筛查的经济有效的方法。光致化学发光试验（LiCA）无需洗涤，只需少量血清，并支持灵活的组合检测过敏原。方法：基于LiCA技术不需要洗涤的优点，本研究建立了“4 + 3× + 1”过敏原检测模式。抗原包被在化学珠上，化学珠选择性地加入检测孔中，实现灵活组合，支持同时检测8种过敏原。结果：本研究引入了一种新的过敏原筛选模型“4 + 3x + 1”，其中“1”代表tIgE，“4”包含四种常见的室内可吸入过敏原，“3x”包含多种常见的可吸入过敏原。“3x”过敏原可根据地区特点和患者具体情况选择。对“4-Fixed”中4种变应原的中间精密度（3.59 % ~ 9.71 %）和重复性（2.81 % ~ 9.31 %）进行了检测，LoB（0.035 ~ 0.066 kUA/L）、LoD（0.092 ~ 0.156 kUA/L）和LoQ（0.135 ~ 0.199 kUA/L）均取得了较好的结果。我们比较了LiCA和ImmunoCAP/Dot-ELISA的一致性，均大于0.8828。另外，分别从“4-Fixed”和“3×-Optional”中选择2个过敏原进行敏感性（80 % ~ 97.72 %）和特异性（93.75 % ~ 100 %）检测。结论：提出的联合测试模型为过敏原筛查提供了一种新的、经济的、快速的选择，它需要低血容量，并且可以为个体患者定制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of immunological methods 医学-免疫学

CiteScore

4.10

自引率

0.00%

发文量

120

审稿时长

3 months

期刊介绍： The Journal of Immunological Methods is devoted to covering techniques for: (1) Quantitating and detecting antibodies and/or antigens. (2) Purifying immunoglobulins, lymphokines and other molecules of the immune system. (3) Isolating antigens and other substances important in immunological processes. (4) Labelling antigens and antibodies. (5) Localizing antigens and/or antibodies in tissues and cells. (6) Detecting, and fractionating immunocompetent cells. (7) Assaying for cellular immunity. (8) Documenting cell-cell interactions. (9) Initiating immunity and unresponsiveness. (10) Transplanting tissues. (11) Studying items closely related to immunity such as complement, reticuloendothelial system and others. (12) Molecular techniques for studying immune cells and their receptors. (13) Imaging of the immune system. (14) Methods for production or their fragments in eukaryotic and prokaryotic cells. In addition the journal will publish articles on novel methods for analysing the organization, structure and expression of genes for immunologically important molecules such as immunoglobulins, T cell receptors and accessory molecules involved in antigen recognition, processing and presentation. Submitted full length manuscripts should describe new methods of broad applicability to immunology and not simply the application of an established method to a particular substance - although papers describing such applications may be considered for publication as a short Technical Note. Review articles will also be published by the Journal of Immunological Methods. In general these manuscripts are by solicitation however anyone interested in submitting a review can contact the Reviews Editor and provide an outline of the proposed review.