4D-FastDIA proteomic analysis of pedal mucus in Pacific abalone Haliotis discus hannai heat-resistance group

IF 2.2 2区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-03-31 DOI:10.1016/j.cbd.2025.101503

Guilan Di , Yu Zhang , Mingmei Jiang , Weini Zhang , Yunlong Wu , Zeyuan Ma , Weilong Yang , Yuqin Fu , Xinhua Chen

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引用次数: 0

Abstract

This study aimed to investigate the immune mechanisms of pedal mucus in Pacific abalone Haliotis discus hannai from different populations. Proteomic differences between the pedal mucus of the control group and the heat-resistance group of H. discus hannai were comparatively analyzed, and the proteins were annotated and analyzed to understand the related functions and roles of differentially expressed proteins (DEPs). The results showed that there were 4054 DEPs in total, among which the DEPs in the pedal mucus of the heat-resistance group and the control group were mainly immune-related heat shock proteins, calmodulin, Ig-like and fibronectin type III domain-containing proteins, histones and mucins, etc.; the DEPs related to growth metabolism included glutathione, growth factor receptor-bound protein, alanine aminotransferase, etc. Circadian entrainment signaling pathway and growth hormone synthesis, secretion and action signaling pathway was significantly enriched, cortisol synthesis and secretion signaling pathway was up-regulated in Haliotis discus hannai heat-resistance group. This study provided references for exploring the relationship between the mucus proteome difference in pedal mucus from different abalone populations sources. These results would contribute to further search for proteins related to immunity, growth and stress resistance, and provide theoretical basis for the development of high-quality germplasm resources of Pacific abalone.

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太平洋鲍鱼足部黏液的4D-FastDIA蛋白组学分析

本研究旨在探讨不同种群太平洋鲍鱼足部黏液的免疫机制。比较分析对照组和耐热组铁饼麻足部黏液的蛋白质组学差异，并对蛋白质进行注释分析，了解差异表达蛋白（differentially expressed proteins, DEPs）的相关功能和作用。结果显示，dep共4054个，其中耐热组和对照组足部粘液中的dep主要为免疫相关热休克蛋白、钙调蛋白、igg样蛋白和纤维连接蛋白III型结构域蛋白、组蛋白和粘蛋白等；与生长代谢相关的DEPs包括谷胱甘肽、生长因子受体结合蛋白、丙氨酸转氨酶等。生理昼夜干扰信号通路和生长激素合成、分泌和作用信号通路显著富集，皮质醇合成和分泌信号通路上调。本研究为探索不同种群来源鲍鱼足部粘液蛋白组差异之间的关系提供了参考。这些结果将有助于进一步寻找与免疫、生长和抗逆性相关的蛋白质，并为开发太平洋鲍鱼优质种质资源提供理论依据。

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来源期刊

Comparative Biochemistry and Physiology D-Genomics & Proteomics 生物-生化与分子生物学

CiteScore

5.10

自引率

3.30%

发文量

审稿时长

33 days

期刊介绍： Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology. Part D: Genomics and Proteomics (CBPD), focuses on “omics” approaches to physiology, including comparative and functional genomics, metagenomics, transcriptomics, proteomics, metabolomics, and lipidomics. Most studies employ “omics” and/or system biology to test specific hypotheses about molecular and biochemical mechanisms underlying physiological responses to the environment. We encourage papers that address fundamental questions in comparative physiology and biochemistry rather than studies with a focus that is purely technical, methodological or descriptive in nature.