Green High-Performance Thin-Layer Chromatography: A Step Towards Eco-Friendly Analysis

IF 1.2 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Chromatographia Pub Date : 2025-03-14 DOI:10.1007/s10337-025-04397-5

Swecha Singh, Nazish khan, Tanushree Sawant, Radhika Raheja

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引用次数: 0

Abstract

High-performance thin-layer chromatography (HPTLC) is a simple but reliable, sensitive, economical, and environmentally benign analytical technique for pharmaceuticals and herbal samples. Its advantages towards quantitative analysis include accurate sample application, quicker and efficient resolution of mixtures, minimum solvent usage and reduced sample size. The ability of HPTLC to examine numerous samples of varying complexity simultaneously and in parallel sets it apart from HPLC. This allows for a high sample throughput and the consumption of solvent and energy is low per sample. Furthermore, compared to HPLC, the use of HPTLC can conserve environmental resources because it requires little to no sample pre-treatments such as liquid–liquid extraction or solid-phase extraction. Several research studies on analytical method development using HPTLC now focus on the use of green solvents in lieu of traditional organic solvents, with the aim to reduce environmental pollution without compromising on analytical performance. In addition, advancements in assessment tools for determining “greenness”, such as AGREE (Analytical GREEnness metric approach and software), GAPI, NEMI, facilitate the evaluation of greenness for specific analysis. The green methods continue to offer rapid, sensitive, and environmentally friendly methodologies, complying with international guidelines of analytical techniques. Overall, the integration of green solvent-based HPTLC methods into analytical workflows represents a significant step towards sustainable and environmentally conscious analytical practices.

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来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.