Green High-Performance Thin-Layer Chromatography: A Step Towards Eco-Friendly Analysis

IF 1.2 4区 化学 Q4 BIOCHEMICAL RESEARCH METHODS
Swecha Singh, Nazish khan, Tanushree Sawant, Radhika Raheja
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引用次数: 0

Abstract

High-performance thin-layer chromatography (HPTLC) is a simple but reliable, sensitive, economical, and environmentally benign analytical technique for pharmaceuticals and herbal samples. Its advantages towards quantitative analysis include accurate sample application, quicker and efficient resolution of mixtures, minimum solvent usage and reduced sample size. The ability of HPTLC to examine numerous samples of varying complexity simultaneously and in parallel sets it apart from HPLC. This allows for a high sample throughput and the consumption of solvent and energy is low per sample. Furthermore, compared to HPLC, the use of HPTLC can conserve environmental resources because it requires little to no sample pre-treatments such as liquid–liquid extraction or solid-phase extraction. Several research studies on analytical method development using HPTLC now focus on the use of green solvents in lieu of traditional organic solvents, with the aim to reduce environmental pollution without compromising on analytical performance. In addition, advancements in assessment tools for determining “greenness”, such as AGREE (Analytical GREEnness metric approach and software), GAPI, NEMI, facilitate the evaluation of greenness for specific analysis. The green methods continue to offer rapid, sensitive, and environmentally friendly methodologies, complying with international guidelines of analytical techniques. Overall, the integration of green solvent-based HPTLC methods into analytical workflows represents a significant step towards sustainable and environmentally conscious analytical practices.

Graphical Abstract

绿色高效薄层色谱:迈向环保分析的一步
高效薄层色谱法(HPTLC)是一种简单、可靠、灵敏、经济、环保的药物和草药样品分析技术。它对定量分析的优点包括准确的样品应用,更快和有效的混合物分辨率,最小的溶剂使用和减少样本量。HPTLC能够同时和平行地检查许多不同复杂程度的样品,这使它有别于HPLC。这允许高样品吞吐量和溶剂和能量的消耗是低每个样品。此外,与HPLC相比,使用HPTLC几乎不需要液-液萃取或固相萃取等样品预处理,可以节约环境资源。目前,关于HPTLC分析方法开发的一些研究侧重于使用绿色溶剂代替传统的有机溶剂,目的是在不影响分析性能的情况下减少环境污染。此外,用于确定“绿色”的评估工具的进步,如AGREE(分析绿色度量方法和软件),GAPI, NEMI,促进了绿色评估的具体分析。绿色方法继续提供快速、敏感和环保的方法,符合国际分析技术准则。总的来说,将绿色溶剂型HPTLC方法整合到分析工作流程中是朝着可持续和环保的分析实践迈出的重要一步。图形抽象
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来源期刊
Chromatographia
Chromatographia 化学-分析化学
CiteScore
3.40
自引率
5.90%
发文量
103
审稿时长
2.2 months
期刊介绍: Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.
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