Effect of Sudan Black B on the Separation and Characterization of Serum Lipoproteins by Asymmetrical Flow Field-Flow Fractionation

IF 1.2 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Chromatographia Pub Date : 2025-02-08 DOI:10.1007/s10337-025-04393-9

Chenjing Zhang, Xiaoyue Zhang, Kaiming Yang, Jing Zhang, Xiaodong Dong, Haiyang Dou

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Abstract

Coronary artery disease (CAD) is a very common cardiovascular disease. Clinically, the content of low-density lipoprotein-cholesterol (LDL-C) is often used as a predictor of CAD. However, severe CAD has been observed in patients with normal level of LDL-C. In this study, the serum lipoproteins stained with Sudan Black B (SBB) were separated and characterized by using asymmetrical flow field-flow fractionation (AF4) coupled with ultraviolet visible (UV/Vis) detector (AF4-UV/Vis). The effects of SBB staining conditions and AF4 operation conditions on AF4 analysis of serum lipoproteins were systematically investigated. The membrane fouling was alleviated by adjusting SBB staining conditions, which enhances the repeatability and accuracy of AF4 analysis of serum lipoproteins. Moreover, the baseline separation of LDL and high-density lipoprotein (HDL) was obtained by AF4 under optimized operation conditions. The results showed that the LDL/HDL value of healthy people was lower than that of CAD patients. The results revealed that the combination of AF4-UV/Vis and SBB staining is a promising approach for the separation and characterization of serum lipoproteins, which is beneficial to screening potential predictors of CAD.

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苏丹黑B对不对称流场-流分馏法分离和表征血清脂蛋白的影响

冠状动脉疾病（CAD）是一种非常常见的心血管疾病。临床上，低密度脂蛋白-胆固醇（LDL-C）的含量常被用作CAD的预测指标。然而，在LDL-C水平正常的患者中观察到严重的CAD。本研究采用不对称流场-流分馏(AF4) -紫外可见（UV/Vis）检测器（AF4-UV/Vis）对苏丹黑B （SBB）染色的血清脂蛋白进行分离和表征。系统探讨SBB染色条件和AF4操作条件对血清脂蛋白AF4分析的影响。通过调整SBB染色条件减轻了膜污染，提高了AF4分析血清脂蛋白的重复性和准确性。同时，在优化的操作条件下，用AF4获得了LDL和高密度脂蛋白（HDL）的基线分离。结果显示，健康人LDL/HDL值低于冠心病患者。结果表明，AF4-UV/Vis联合SBB染色是一种很有前途的分离和表征血清脂蛋白的方法，有助于筛选CAD的潜在预测因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.