A Comparison between LTT and CFSE Proliferation Tests in Patients with Inborn Errors of Adaptive Immunity.

Endocrine, metabolic & immune disorders drug targets Pub Date : 2025-04-03 DOI:10.2174/0118715303363026250312063731

Tannaz Moeini Shad, Nima Taghizadeh, Samaneh Delavari, Nazanin Aghamohamadi, Ali Zarezadeh Mehrabadi, Fereshte Salami, Soha Mohammadi, Helia Salehi, Saba Fekrvand, Marzieh Tavakol, Nazanin Fathi, Zahra Chavoshzadeh, Mehrnaz Mesdaghi, Mahsa Ghadrdan, Alireza Shafiei, Nima Rezaei, Reza Yazdani, Hassan Abolhassani

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Abstract

Background: Inborn errors of immunity (IEI) include immunodeficiencies affecting cellular and humoral immunity.

Objectives: We aimed to compare the effectiveness of the LTT and carboxyfluorescein succinimidyl ester (CFSE) assays in assessing lymphocyte proliferation in IEI patients.

Methods: We utilized radioactive [3H]-thymidine and non-radioactive CFSE to measure lymphocyte proliferation in three distinct groups: syndromic CID (SyCID), non-syndromic combined immunodeficiency (N-SyCID), and primary antibody deficiency (PAD).

Results: LTT identified 8 cases of abnormal lymphocyte proliferation among all patients, whereas CFSE detected 23 cases. In the N-SyCID group, LTT identified more abnormalities than CFSE, whereas, in the SyCID and PAD groups, CFSE detected more defects. Two patients with ataxia telangiectasia and CVID had positive results on both LTT and CFSE tests, and a specific ORAI1 gene mutation resulted in differing test outcomes.

Conclusion: It was found that the CFSE method is a reliable and practical choice for measuring mitogenic T-cell responses in unclassified IEI patients for confirmation of immunologic diagnosis.

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先天性适应性免疫错误患者LTT与CFSE增殖试验的比较

背景：先天性免疫错误（IEI）包括影响细胞和体液免疫的免疫缺陷。目的：我们旨在比较LTT和羧荧光素琥珀酰酰酯（CFSE）测定在评估IEI患者淋巴细胞增殖方面的有效性。方法：利用放射性[3H]-胸腺嘧啶和非放射性CFSE检测三种不同组：综合征型CID （SyCID）、非综合征型联合免疫缺陷（N-SyCID）和一抗缺乏症（PAD）的淋巴细胞增殖。结果：LTT检出淋巴细胞增生异常8例，CFSE检出23例。在N-SyCID组中，LTT比CFSE检测到更多的异常，而在SyCID和PAD组中，CFSE检测到更多的缺陷。两例共济失调毛细血管扩张和CVID患者在LTT和CFSE检测中均呈阳性结果，特异性ORAI1基因突变导致检测结果不同。结论：CFSE法是一种可靠、实用的方法，可用于检测未分类IEI患者的有丝分裂性t细胞反应，以确定免疫诊断。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Endocrine, metabolic & immune disorders drug targets

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