Integrative taxonomy and DNA barcoding of Thai Caddisflies (Trichoptera), with the description of a new Species.

IF 0.8 4区生物学 Q3 ZOOLOGY

Zootaxa Pub Date : 2025-03-27 DOI:10.11646/zootaxa.5613.2.6

Chavanut Jaroenchaiwattanachote, Pairot Pramual, Khanit Wangwasit, Pasakorn Bunchalee, Isara Thanee

{"title":"Integrative taxonomy and DNA barcoding of Thai Caddisflies (Trichoptera), with the description of a new Species.","authors":"Chavanut Jaroenchaiwattanachote, Pairot Pramual, Khanit Wangwasit, Pasakorn Bunchalee, Isara Thanee","doi":"10.11646/zootaxa.5613.2.6","DOIUrl":null,"url":null,"abstract":"<p><p>Caddisflies (Trichoptera) are abundant and diverse aquatic insects. Their immature stages inhabit a wide range of aquatic environments, making them ideal candidates for water quality biomonitoring. However, the limited morphological characteristics available for species identification in the immature stages pose a significant challenge to their application in biomonitoring. In this study, we evaluated the effectiveness of DNA barcoding, based on mitochondrial cytochrome c oxidase I (COI) sequences, for species identification of caddisflies in Thailand. A total of 1,487 adult specimens were collected and morphologically identified into 13 species across 8 genera and 4 families. From these taxa, 88 COI sequences were generated from representative specimens. Maximum intraspecific genetic divergence ranged from 0% to 3.08%. Only three species were successfully matched to COI sequences in the BOLD database, while nine species are reported here for the first time, and one species remained ambiguous. Integrating COI barcoding sequences with morphological data revealed that one species, morphologically similar to Triplectides indicus (Walker 1852), represents a novel species, Triplectides buengkanensis sp. nov. We provide a detailed description, illustrations, diagnostic features, and DNA barcoding sequences for this new species.</p>","PeriodicalId":24072,"journal":{"name":"Zootaxa","volume":"5613 2","pages":"307-322"},"PeriodicalIF":0.8000,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zootaxa","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.11646/zootaxa.5613.2.6","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ZOOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Caddisflies (Trichoptera) are abundant and diverse aquatic insects. Their immature stages inhabit a wide range of aquatic environments, making them ideal candidates for water quality biomonitoring. However, the limited morphological characteristics available for species identification in the immature stages pose a significant challenge to their application in biomonitoring. In this study, we evaluated the effectiveness of DNA barcoding, based on mitochondrial cytochrome c oxidase I (COI) sequences, for species identification of caddisflies in Thailand. A total of 1,487 adult specimens were collected and morphologically identified into 13 species across 8 genera and 4 families. From these taxa, 88 COI sequences were generated from representative specimens. Maximum intraspecific genetic divergence ranged from 0% to 3.08%. Only three species were successfully matched to COI sequences in the BOLD database, while nine species are reported here for the first time, and one species remained ambiguous. Integrating COI barcoding sequences with morphological data revealed that one species, morphologically similar to Triplectides indicus (Walker 1852), represents a novel species, Triplectides buengkanensis sp. nov. We provide a detailed description, illustrations, diagnostic features, and DNA barcoding sequences for this new species.

查看原文本刊更多论文

笛蝇属（毛翅目）是一种数量丰富、种类繁多的水生昆虫。它们的未成熟阶段栖息于广泛的水生环境中，是水质生物监测的理想候选对象。然而，未成熟阶段用于物种鉴定的形态特征有限，这给它们在生物监测中的应用带来了巨大挑战。在本研究中，我们评估了基于线粒体细胞色素 c 氧化酶 I（COI）序列的 DNA 条形码在泰国蝶类物种鉴定中的有效性。研究人员共采集了 1,487 个成年标本，并通过形态学鉴定为 8 属 4 科 13 种。从这些类群中，有代表性的标本产生了 88 条 COI 序列。种内最大遗传差异从 0% 到 3.08% 不等。只有 3 个物种与 BOLD 数据库中的 COI 序列成功匹配，9 个物种为首次报道，1 个物种仍不明确。将 COI 条形码序列与形态学数据整合后发现，有一个物种与 Triplectides indicus（Walker 1852）形态相似，是一个新物种，即 Triplectides buengkanensis sp.我们提供了该新种的详细描述、插图、诊断特征和 DNA 条形码序列。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Zootaxa 生物-动物学

CiteScore

1.90

自引率

33.30%

发文量

1895

审稿时长

2.7 months

期刊介绍： Zootaxa is a peer-reviewed international journal for rapid publication of high quality papers on any aspect of systematic zoology, with a preference for large taxonomic works such as monographs and revisions. Zootaxa considers papers on all animal taxa, both living and fossil, and especially encourages descriptions of new taxa. All types of taxonomic papers are considered, including theories and methods of systematics and phylogeny, taxonomic monographs, revisions and reviews, catalogues/checklists, biographies and bibliographies, identification guides, analysis of characters, phylogenetic relationships and zoogeographical patterns of distribution, descriptions of taxa, and nomenclature. Open access publishing option is strongly encouraged for authors with research grants and other funds. For those without grants/funds, all accepted manuscripts will be published but access is secured for subscribers only.