Katja K Koll, Donna A Klevansky, Dimitri Kasakovski, Nima Ahmadzadeh, Patrick A Will, Ulrich Kneser, Christoph Hirche
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引用次数: 0
Abstract
Background: Regenerative matrices are essential in the therapy of complex wounds, where lymphangiogenesis and angiogenesis play crucial roles. This work investigates the lymph- and angiogenic potential of regenerative scaffolds and the influence of adipose-derived mesenchymal stem cells (ADSC) in a rodent wound model.
Methods: In a wound model, we administered different Acellular Dermal Matrices (ADMs) to two wounds on each rat-enriching one with ADSCs and leaving the other without. ADSCs were isolated from rodent inguinal fat tissue, characterized using FACS, and their viability confirmed in vitro on different matrices. Tissue samples were collected on days 7, 14, 21, and 28 postoperatively. Six different ADMs, including two synthetic and four biological variants, were compared using histological, immunofluorescence staining, and molecular biological analyses.
Results: ADM integration varied, with Collagen-Elastin-ADM showing the highest integration (95%) and Polyurethane-ADM the lowest (23%) after 7 days. Synthetic ADMs, particularly Polyurethan-ADM, exhibited the highest mRNA expression of angiogenesis and lymphangiogenesis markers at 7 and 28 days. Protein analysis showed synthetic ADMs had the highest CD31 levels from 14 to 28 days, while Collagen-ADM had the highest LYVE1 and PROX1 levels at 21 and 28 days. Immunofluorescence revealed Polylactide-ADM consistently had the highest CD31 and LYVE1 signals at various times, with Polyurethan-ADM and Collagen-ADM peaking at 28 days. The application of ADSCs did not enhance lymphangiogenesis or angiogenesis.
Conclusion and clinical relevance statement: ADM material properties impact lymphangiogenesis and angiogenesis, guiding therapeutic selection and tissue regeneration. This study highlights Collagen-, Polylactide-, and Polyurethane-ADMs as the most effective in promoting these processes.
期刊介绍：
For more than 70 years Plastic and Reconstructive Surgery® has been the one consistently excellent reference for every specialist who uses plastic surgery techniques or works in conjunction with a plastic surgeon. Plastic and Reconstructive Surgery® , the official journal of the American Society of Plastic Surgeons, is a benefit of Society membership, and is also available on a subscription basis.
Plastic and Reconstructive Surgery® brings subscribers up-to-the-minute reports on the latest techniques and follow-up for all areas of plastic and reconstructive surgery, including breast reconstruction, experimental studies, maxillofacial reconstruction, hand and microsurgery, burn repair, cosmetic surgery, as well as news on medicolegal issues. The cosmetic section provides expanded coverage on new procedures and techniques and offers more cosmetic-specific content than any other journal. All subscribers enjoy full access to the Journal''s website, which features broadcast quality videos of reconstructive and cosmetic procedures, podcasts, comprehensive article archives dating to 1946, and additional benefits offered by the newly-redesigned website.
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