Semitendinosus tendons in physically immature patients have a lower type I collagen content.

IF 3.3 2区医学 Q1 ORTHOPEDICS

Knee Surgery, Sports Traumatology, Arthroscopy Pub Date : 2025-04-03 DOI:10.1002/ksa.12673

Yushin Mizuno, Yasushi Takata, Kazuaki Yoshioka, Naoki Takemoto, Manase Nishimura, Kentaro Fujita, Takuya Sengoku, Sakae Miyagi, Satoru Demura, Junsuke Nakase

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引用次数: 0

Abstract

Purpose: To determine the collagen composition of the semitendinosus and quadriceps tendons at different levels of physical maturity.

Methods: Tissue samples were collected from 70 patients who underwent anterior cruciate ligament or medial patellofemoral ligament reconstruction using the semitendinosus or quadriceps tendon. The samples were immunostained to identify type I and type III collagen. Confocal microscopy and image analysis software were used to determine the percentage composition of collagen types (type I + type III, 100%). Patients were categorized into three groups based on age and epiphyseal patency: immature (<20 years, >1.5 mm), young (<20 years, <1.5 mm) and adult (>20 years). The Mann-Whitney U test was used to analyze differences between the immature and other groups in the semitendinosus tendon group. The percentage of collagen content in the semitendinosus and quadriceps tendons was evaluated based on the patient's maturity level.

Results: The median type I collagen content in the semitendinosus tendon was 54.2% (40.9-75.4), 98.9% (81.0-99.6) and 97.0% (40.0-100.0) in the immature (n = 6), young (n = 8) and adult (n = 18) groups, respectively. For the quadriceps tendon, the values were 98.4% (68.9-100.0), 89.1% (68.6-98.9) and 85.6% (72.3-99.6) in the immature (n = 11), young (n = 14) and adult (n = 13) groups, respectively. The semitendinosus tendon in the immature group had significantly lower type I collagen content than in the young and adult groups. Additionally, in the immature group, type I collagen content was significantly lower in the semitendinosus tendon than in the quadriceps tendon.

Conclusions: A comparison of collagen composition between the semitendinosus and quadriceps tendons, based on patient maturity level, revealed that the semitendinosus tendon in immature patients had a lower percentage of type I collagen than in mature patients and lower than the quadriceps tendon.

Level of evidence: Not applicable.

查看原文本刊更多论文

身体发育不成熟患者的半腱肌腱 I 型胶原蛋白含量较低。

目的：测定半腱肌和股四头肌不同生理成熟水平的胶原组成。方法：对70例采用半腱肌或股四头肌肌腱重建髌股前交叉韧带或髌股内侧韧带的患者进行组织标本收集。对样品进行免疫染色以鉴定I型和III型胶原蛋白。使用共聚焦显微镜和图像分析软件确定胶原类型的百分比组成（I型+ III型，100%）。患者根据年龄和骨骺通畅程度分为三组：未成熟（1.5 mm），年轻（20岁）。采用Mann-Whitney U检验分析半腱肌腱组未成熟组与其他组之间的差异。根据患者的成熟程度评估半腱肌和股四头肌肌腱中胶原含量的百分比。结果：未成熟组（n = 6）、幼龄组（n = 8）、成年组（n = 18）半腱肌肌腱中I型胶原含量中位数分别为54.2%（40.9 ~ 75.4）、98.9%（81.0 ~ 99.6）、97.0%（40.0 ~ 100.0）。在未成熟组（n = 11）、幼龄组（n = 14）和成年组（n = 13）中，股四头肌肌腱的阳性率分别为98.4%（68.9-100.0）、89.1%（68.6-98.9）和85.6%（72.3-99.6）。未成熟组半腱肌腱I型胶原含量明显低于青壮年组和成年组。此外，在未成熟组中，半腱肌肌腱中的I型胶原含量明显低于股四头肌肌腱。结论：根据患者成熟程度对半腱肌肌腱和股四头肌肌腱的胶原组成进行比较，发现未成熟患者的半腱肌肌腱的I型胶原百分比低于成熟患者，低于股四头肌肌腱。证据等级：不适用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Knee Surgery, Sports Traumatology, Arthroscopy 医学-外科

CiteScore

8.10

自引率

18.40%

发文量

418

审稿时长

2 months

期刊介绍： Few other areas of orthopedic surgery and traumatology have undergone such a dramatic evolution in the last 10 years as knee surgery, arthroscopy and sports traumatology. Ranked among the top 33% of journals in both Orthopedics and Sports Sciences, the goal of this European journal is to publish papers about innovative knee surgery, sports trauma surgery and arthroscopy. Each issue features a series of peer-reviewed articles that deal with diagnosis and management and with basic research. Each issue also contains at least one review article about an important clinical problem. Case presentations or short notes about technical innovations are also accepted for publication. The articles cover all aspects of knee surgery and all types of sports trauma; in addition, epidemiology, diagnosis, treatment and prevention, and all types of arthroscopy (not only the knee but also the shoulder, elbow, wrist, hip, ankle, etc.) are addressed. Articles on new diagnostic techniques such as MRI and ultrasound and high-quality articles about the biomechanics of joints, muscles and tendons are included. Although this is largely a clinical journal, it is also open to basic research with clinical relevance. Because the journal is supported by a distinguished European Editorial Board, assisted by an international Advisory Board, you can be assured that the journal maintains the highest standards. Official Clinical Journal of the European Society of Sports Traumatology, Knee Surgery and Arthroscopy (ESSKA).