{"title":"Semitendinosus tendons in physically immature patients have a lower type I collagen content.","authors":"Yushin Mizuno, Yasushi Takata, Kazuaki Yoshioka, Naoki Takemoto, Manase Nishimura, Kentaro Fujita, Takuya Sengoku, Sakae Miyagi, Satoru Demura, Junsuke Nakase","doi":"10.1002/ksa.12673","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>To determine the collagen composition of the semitendinosus and quadriceps tendons at different levels of physical maturity.</p><p><strong>Methods: </strong>Tissue samples were collected from 70 patients who underwent anterior cruciate ligament or medial patellofemoral ligament reconstruction using the semitendinosus or quadriceps tendon. The samples were immunostained to identify type I and type III collagen. Confocal microscopy and image analysis software were used to determine the percentage composition of collagen types (type I + type III, 100%). Patients were categorized into three groups based on age and epiphyseal patency: immature (<20 years, >1.5 mm), young (<20 years, <1.5 mm) and adult (>20 years). The Mann-Whitney U test was used to analyze differences between the immature and other groups in the semitendinosus tendon group. The percentage of collagen content in the semitendinosus and quadriceps tendons was evaluated based on the patient's maturity level.</p><p><strong>Results: </strong>The median type I collagen content in the semitendinosus tendon was 54.2% (40.9-75.4), 98.9% (81.0-99.6) and 97.0% (40.0-100.0) in the immature (n = 6), young (n = 8) and adult (n = 18) groups, respectively. For the quadriceps tendon, the values were 98.4% (68.9-100.0), 89.1% (68.6-98.9) and 85.6% (72.3-99.6) in the immature (n = 11), young (n = 14) and adult (n = 13) groups, respectively. The semitendinosus tendon in the immature group had significantly lower type I collagen content than in the young and adult groups. Additionally, in the immature group, type I collagen content was significantly lower in the semitendinosus tendon than in the quadriceps tendon.</p><p><strong>Conclusions: </strong>A comparison of collagen composition between the semitendinosus and quadriceps tendons, based on patient maturity level, revealed that the semitendinosus tendon in immature patients had a lower percentage of type I collagen than in mature patients and lower than the quadriceps tendon.</p><p><strong>Level of evidence: </strong>Not applicable.</p>","PeriodicalId":17880,"journal":{"name":"Knee Surgery, Sports Traumatology, Arthroscopy","volume":" ","pages":""},"PeriodicalIF":3.3000,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Knee Surgery, Sports Traumatology, Arthroscopy","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1002/ksa.12673","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ORTHOPEDICS","Score":null,"Total":0}
引用次数: 0
Abstract
Purpose: To determine the collagen composition of the semitendinosus and quadriceps tendons at different levels of physical maturity.
Methods: Tissue samples were collected from 70 patients who underwent anterior cruciate ligament or medial patellofemoral ligament reconstruction using the semitendinosus or quadriceps tendon. The samples were immunostained to identify type I and type III collagen. Confocal microscopy and image analysis software were used to determine the percentage composition of collagen types (type I + type III, 100%). Patients were categorized into three groups based on age and epiphyseal patency: immature (<20 years, >1.5 mm), young (<20 years, <1.5 mm) and adult (>20 years). The Mann-Whitney U test was used to analyze differences between the immature and other groups in the semitendinosus tendon group. The percentage of collagen content in the semitendinosus and quadriceps tendons was evaluated based on the patient's maturity level.
Results: The median type I collagen content in the semitendinosus tendon was 54.2% (40.9-75.4), 98.9% (81.0-99.6) and 97.0% (40.0-100.0) in the immature (n = 6), young (n = 8) and adult (n = 18) groups, respectively. For the quadriceps tendon, the values were 98.4% (68.9-100.0), 89.1% (68.6-98.9) and 85.6% (72.3-99.6) in the immature (n = 11), young (n = 14) and adult (n = 13) groups, respectively. The semitendinosus tendon in the immature group had significantly lower type I collagen content than in the young and adult groups. Additionally, in the immature group, type I collagen content was significantly lower in the semitendinosus tendon than in the quadriceps tendon.
Conclusions: A comparison of collagen composition between the semitendinosus and quadriceps tendons, based on patient maturity level, revealed that the semitendinosus tendon in immature patients had a lower percentage of type I collagen than in mature patients and lower than the quadriceps tendon.
期刊介绍：
Few other areas of orthopedic surgery and traumatology have undergone such a dramatic evolution in the last 10 years as knee surgery, arthroscopy and sports traumatology. Ranked among the top 33% of journals in both Orthopedics and Sports Sciences, the goal of this European journal is to publish papers about innovative knee surgery, sports trauma surgery and arthroscopy. Each issue features a series of peer-reviewed articles that deal with diagnosis and management and with basic research. Each issue also contains at least one review article about an important clinical problem. Case presentations or short notes about technical innovations are also accepted for publication.
The articles cover all aspects of knee surgery and all types of sports trauma; in addition, epidemiology, diagnosis, treatment and prevention, and all types of arthroscopy (not only the knee but also the shoulder, elbow, wrist, hip, ankle, etc.) are addressed. Articles on new diagnostic techniques such as MRI and ultrasound and high-quality articles about the biomechanics of joints, muscles and tendons are included. Although this is largely a clinical journal, it is also open to basic research with clinical relevance.
Because the journal is supported by a distinguished European Editorial Board, assisted by an international Advisory Board, you can be assured that the journal maintains the highest standards.
Official Clinical Journal of the European Society of Sports Traumatology, Knee Surgery and Arthroscopy (ESSKA).
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