Self-Assembled Fluorophore-Based Probe for Efficient Detection of Endogenous Nitroreductase Activity in Escherichia Coli (Advanced Optical Materials 10/2025)

IF 8 2区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

Advanced Optical Materials Pub Date : 2025-04-04 DOI:10.1002/adom.202570076

Eiji Nakata, Yoshihiro Yukimachi, Hirokazu Kariyazono, Yoshijiro Nazumi, Futa Komatsubara, Mashal Asif, Yoshihiro Uto, Hitoshi Hori

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Abstract

Stimuli-Responsive Supramolecular Cluster Based Fluorescent Probe

A rational design strategy to construct stimuli-responsive supramolecular cluster based fluorescent probes, called “self-assembly induced lactone formation” (SAILac), has been developed. A new nitroreductase-reactive fluorescent probe, Rhodol-OBn(pNO₂), is created based on the structure-activiy relationship studies of the fluorophore scaffold, resulting in a superior S/N ratio and higher reactivity toward nitroreductase than the original probe. This new probe successfully detects the activity of endogenous nitroreductase in Escherichia coli. More details can be found in article 2402530 by Eiji Nakata and co-workers.

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基于自组装荧光团的探针高效检测大肠杆菌内源性硝基还原酶活性（先进光学材料10/2025）

基于刺激响应的超分子簇荧光探针提出了一种构建刺激响应超分子簇荧光探针的合理设计策略，称为“自组装诱导内酯形成”（SAILac）。基于对荧光团支架的构效关系研究，制备了一种新型的硝基还原酶反应性荧光探针rhodolo - obn (pNO2)，该探针具有比原探针更好的S/N比和对硝基还原酶的反应性。该探针成功地检测了大肠杆菌内源性硝基还原酶的活性。更多细节可以在Eiji Nakata及其同事的文章2402530中找到。

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来源期刊

Advanced Optical Materials MATERIALS SCIENCE, MULTIDISCIPLINARY-OPTICS

CiteScore

13.70

自引率

6.70%

发文量

883

审稿时长

1.5 months

期刊介绍： Advanced Optical Materials, part of the esteemed Advanced portfolio, is a unique materials science journal concentrating on all facets of light-matter interactions. For over a decade, it has been the preferred optical materials journal for significant discoveries in photonics, plasmonics, metamaterials, and more. The Advanced portfolio from Wiley is a collection of globally respected, high-impact journals that disseminate the best science from established and emerging researchers, aiding them in fulfilling their mission and amplifying the reach of their scientific discoveries.