MiR-486–5p predicts the progression of severe acute pancreatitis by mediating the inflammatory response and ATG7/p38 MAPK pathway

IF 2.3 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

American Journal of the Medical Sciences Pub Date : 2025-03-30 DOI:10.1016/j.amjms.2025.01.011

Yang Wang MD , Qi Ni MD , Shuying Xu MM , Mingli Cui MM , Ruixia Wang MM , Rong Liu MD

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引用次数: 0

Abstract

Background

Acute pancreatitis (AP) is a serious disorder, and is frequently accompanied by shock or organ failure. The study aimed to investigate the predictive value of serum miR-486–5p for the prognosis of SAP patients and the underlying mechanism.

Methods

The concentration of mRNAs was detected by Real-Time PCR reaction. The correlation between miRNA and each scoring system was analyzed via Pearson's correlation analysis. ROC curve was performed for diagnostic value evaluation. The predictive value of miRNA expression in the severity of AP was estimated by logistic regression analysis. HPDE6-C7 cells were treated with cerulein (Cer) to mimic AP in vitro. The cell apoptosis, viability, and inflammatory response were detected by flow cytometry, CCK-8, and ELISA, respectively. The targeting relationship was verified by DLR assay and RIP assay.

Results

The expression of miR-486–5p was elevated in the serum of non-SAP and SAP groups (P < 0.001), which was interconnected with APACHE II, SOFA, and Ranson scores. MiR-486–5p can differentiate SAP patients from non-SAP with the AUC of 0.916, and it was an independent risk for the severity of AP patients. The miR-486–5p/ATG7 axis affected the apoptosis, viability, and inflammatory response of HPDE6-C7 cell models by the p38 MAPK pathway, thus involving the progression of AP.

Conclusions

Serum miR-486–5p may have a certain predictive value for the severity of AP and influence AP development through mediating cell inflammatory response via targeting ATG7.

查看原文本刊更多论文

MiR-486-5p通过介导炎症反应和ATG7/p38 MAPK通路预测严重急性胰腺炎的进展。

背景：急性胰腺炎（AP）是一种严重的疾病，经常伴有休克或器官衰竭。本研究旨在探讨血清miR-486-5p对SAP患者预后的预测价值及其机制。方法：采用Real-Time PCR法检测mrna的浓度。通过Pearson相关分析分析miRNA与各评分体系的相关性。采用ROC曲线进行诊断价值评价。通过logistic回归分析估计miRNA表达对AP严重程度的预测价值。用cerulein （Cer）处理HPDE6-C7细胞，体外模拟AP。分别用流式细胞术、CCK-8和ELISA检测细胞凋亡、活力和炎症反应。通过DLR法和RIP法验证其靶向关系。结果：miR-486-5p在非SAP组和SAP组血清中表达升高（P < 0.001），且与APACHE II、SOFA、Ranson评分相关。MiR-486-5p能够区分SAP患者与非SAP患者，AUC为0.916，对AP患者的严重程度具有独立风险。miR-486-5p/ATG7轴通过p38 MAPK通路影响HPDE6-C7细胞模型的凋亡、活力和炎症反应，从而参与AP的进展。结论：血清miR-486-5p可能通过靶向ATG7介导细胞炎症反应，对AP的严重程度具有一定的预测价值，影响AP的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American Journal of the Medical Sciences 医学-医学：内科

CiteScore

4.40

自引率

0.00%

发文量

303

审稿时长

1.5 months

期刊介绍： The American Journal of The Medical Sciences (AJMS), founded in 1820, is the 2nd oldest medical journal in the United States. The AJMS is the official journal of the Southern Society for Clinical Investigation (SSCI). The SSCI is dedicated to the advancement of medical research and the exchange of knowledge, information and ideas. Its members are committed to mentoring future generations of medical investigators and promoting careers in academic medicine. The AJMS publishes, on a monthly basis, peer-reviewed articles in the field of internal medicine and its subspecialties, which include: Original clinical and basic science investigations Review articles Online Images in the Medical Sciences Special Features Include: Patient-Centered Focused Reviews History of Medicine The Science of Medical Education.