Effect of Dendritic Cells Injection After Radical Prostatectomy on Prostate Cancer in Mice.

Abstract

Background: To explore the therapeutic and preventive effect of dendritic cells injection combined with radical prostatectomy on prostate cancer in mice.

Methods: We extracted antigens from mouse prostate cancer cells RM-1 and cocultured them with dendritic cells to induce maturation. We constructed in situ carcinoma and subcutaneous tumor models of the mouse prostate. The efficacy of dendritic cell injection combined with radical prostatectomy was evaluated in the carcinoma in situ model, and the ability of dendritic cells to prevent prostate cancer was evaluated in the subcutaneous tumor model. Means of assessment included ultrasonography, flow cytometry analysis, and Elisa.

Results: Dendritic cell injection combined with radical prostatectomy effectively inhibited the growth of prostate carcinoma in situ in mice, as well as the growth of subcutaneous tumors of prostate cancer in mice. After dendritic cell injection, the levels of CD4 + T cells and Treg cells in the spleens of mice were significantly increased, and the levels of IL-2 and TNF-γ in the peripheral serum were significantly increased.

Conclusions: Injection of mature dendritic cells induced by mouse prostate cancer cell RM-1 antigen can inhibit the growth of prostate cancer. Radical prostatectomy combined with dendritic cell injection might be a potential treatment strategy for prostate cancer.