Cryopreservation practices in clinical and preclinical iPSC-based cell therapies: Current challenges and future directions.

IF 2.5 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Progress Pub Date : 2025-04-02 DOI:10.1002/btpr.70031

Michael Dobruskin, Geoffrey Toner, Ronald Kander

引用次数: 0

Abstract

Induced pluripotent stem cells (iPSCs) offer significant therapeutic potential, but cryopreservation challenges, particularly the reliance on cytotoxic Dimethyl Sulfoxide (Me₂SO), hinder their clinical application. This review examines current cryopreservation practices in clinical and preclinical iPSC-based therapies, highlighting the consistent use of Me₂SO and the logistical challenges of post-thaw processing. The findings underscore the urgent need for alternative cryopreservation techniques to ensure the safety and efficacy of off-the-shelf iPSC therapies.

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低温保存在临床和临床前基于ipsc细胞治疗的实践：当前的挑战和未来的方向。

诱导多能干细胞（iPSCs）具有巨大的治疗潜力，但低温保存方面的挑战，特别是对细胞毒性二甲基亚砜（Me2SO）的依赖，阻碍了其临床应用。这篇综述探讨了当前基于 iPSC 的临床和临床前疗法中的冷冻保存实践，强调了 Me2SO 的持续使用和解冻后处理的后勤挑战。研究结果强调了对替代性冷冻保存技术的迫切需要，以确保现成 iPSC 疗法的安全性和有效性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Progress 工程技术-生物工程与应用微生物

CiteScore

6.50

自引率

3.40%

发文量

审稿时长

4 months

期刊介绍： Biotechnology Progress , an official, bimonthly publication of the American Institute of Chemical Engineers and its technological community, the Society for Biological Engineering, features peer-reviewed research articles, reviews, and descriptions of emerging techniques for the development and design of new processes, products, and devices for the biotechnology, biopharmaceutical and bioprocess industries. Widespread interest includes application of biological and engineering principles in fields such as applied cellular physiology and metabolic engineering, biocatalysis and bioreactor design, bioseparations and downstream processing, cell culture and tissue engineering, biosensors and process control, bioinformatics and systems biology, biomaterials and artificial organs, stem cell biology and genetics, and plant biology and food science. Manuscripts concerning the design of related processes, products, or devices are also encouraged. Four types of manuscripts are printed in the Journal: Research Papers, Topical or Review Papers, Letters to the Editor, and R & D Notes.