Integrating Raman spectroscopy and RT-qPCR for enhanced diagnosis of thyroid lesions: A comparative study of biochemical and molecular markers

IF 3.1 3区医学 Q2 CHEMISTRY, ANALYTICAL

Journal of pharmaceutical and biomedical analysis Pub Date : 2025-03-28 DOI:10.1016/j.jpba.2025.116844

Lázaro P.M. Neto , Laurita dos Santos , Luis Felipe C.S. Carvalho , André B.O. Santos , Airton A. Martin , Renata A. Canevari

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引用次数: 0

Abstract

Thyroid cancer is the most prevalent endocrine malignancy, with increasing incidence due to advancements in diagnostic techniques. Ultrasound (US) and fine needle aspiration (FNA) cytology, widely used in clinical practice, have detection accuracies ranging from 65 % to 95 %. However, these methods may yield inconclusive or difficult-to-interpret results, emphasizing the need for complementary diagnostic techniques. This study explores the integration of Raman spectroscopy and gene expression analysis via RT-qPCR to improve the diagnosis of thyroid lesions, classified into groups: follicular thyroid carcinoma (FTC), papillary thyroid carcinoma (PTC) and goiter tissues. Healthy tissue samples were used as normalizing controls in both analysis. Raman spectroscopy analyzed 35 samples, while RT-qPCR assessed 33 samples. For comparison, the same 19 samples previously analyzed by both techniques were examined. Raman spectroscopy, a non-invasive technique, has shown effectiveness in distinguishing between benign and malignant thyroid tissues by identifying key biochemical components such as DNA, RNA, proteins, and lipids. The distinguishing of FTC from goiter using Raman spectroscopy achieved an accuracy rate of 82.3 %. Gene expression analysis via RT-qPCR focused on six genes: TG, TPO, PDGFB, SERPINA1, TFF3, and LGALS3. Specifically, SERPINA1 was overexpressed in PTC, TFF3 showed elevated levels in FTC, and LGALS3 was elevated in both PTC and FTC compared to goiter and normal tissues. These findings align with existing literature, suggesting that these genes could serve as valuable diagnostic molecular markers. The expression analysis of these genes within this subset of samples demonstrated concordance with the classification derived from PCA of Raman spectroscopy data. The integration of Raman spectroscopy and RT-qPCR offers a complementary approach to traditional histological analysis, providing enhanced sensitivity and specificity in diagnosing thyroid lesions.

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来源期刊

Journal of pharmaceutical and biomedical analysis 医学-分析化学

CiteScore

6.70

自引率

5.90%

发文量

588

审稿时长

37 days

期刊介绍： This journal is an international medium directed towards the needs of academic, clinical, government and industrial analysis by publishing original research reports and critical reviews on pharmaceutical and biomedical analysis. It covers the interdisciplinary aspects of analysis in the pharmaceutical, biomedical and clinical sciences, including developments in analytical methodology, instrumentation, computation and interpretation. Submissions on novel applications focusing on drug purity and stability studies, pharmacokinetics, therapeutic monitoring, metabolic profiling; drug-related aspects of analytical biochemistry and forensic toxicology; quality assurance in the pharmaceutical industry are also welcome. Studies from areas of well established and poorly selective methods, such as UV-VIS spectrophotometry (including derivative and multi-wavelength measurements), basic electroanalytical (potentiometric, polarographic and voltammetric) methods, fluorimetry, flow-injection analysis, etc. are accepted for publication in exceptional cases only, if a unique and substantial advantage over presently known systems is demonstrated. The same applies to the assay of simple drug formulations by any kind of methods and the determination of drugs in biological samples based merely on spiked samples. Drug purity/stability studies should contain information on the structure elucidation of the impurities/degradants.