Neoehrlichia mikurensis: an emerging pathogen in Southeastern Poland - prevalence in Ixodes ricinus ticks and phylogenetic characterization.

IF 1.3 4区医学 Q4 ENVIRONMENTAL SCIENCES

Annals of Agricultural and Environmental Medicine Pub Date : 2025-03-25 Epub Date: 2025-01-10 DOI:10.26444/aaem/199643

Zbigniew Zając, Joanna Kulisz, Renata Kunc-Kozioł, Aneta Woźniak, Angelique Foucault-Simonin, Pavle Banović, Alexandra Corduneanu, Sara Moutailler, Alejandro Cabezas-Cruz

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引用次数: 0

Abstract

Introduction: Neoehrlichia mikurensis is an emerging pathogen increasingly detected in ticks, animals, and humans. The study aimed to investigate the presence and phylogenetic diversity of Ixodes ricinus ticks in the Subcarpathian Region.

Material and methods: Of the 412 ticks collected from forest and meadow habitats, 240 specimens underwent molecular analysis for presence of N. mikurensis using microfluidic real-time PCR. Selected amplicons were sequenced to preform phylogenetic analysis of the groEL gene.

Results: N. mikurensis infection was confirmed in 6.35% of female and 2.38% of male ticks, with no significant differences in the prevalence between habitats. Phylogenetic analysis revealed a low genetic diversity N. mikurensis.

Conclusions: The findings confirm the infection of I. ricinus ticks with N. mikurensis in the studied region. The studied N. mikurensis groEL gene sequences showed low genetic variation, identical to other populations in Poland and Europe.

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来源期刊

Annals of Agricultural and Environmental Medicine 医学-公共卫生、环境卫生与职业卫生

CiteScore

3.00

自引率

5.90%

发文量

审稿时长

4-8 weeks

期刊介绍： All papers within the scope indicated by the following sections of the journal may be submitted: Biological agents posing occupational risk in agriculture, forestry, food industry and wood industry and diseases caused by these agents (zoonoses, allergic and immunotoxic diseases). Health effects of chemical pollutants in agricultural areas , including occupational and non-occupational effects of agricultural chemicals (pesticides, fertilizers) and effects of industrial disposal (heavy metals, sulphur, etc.) contaminating the atmosphere, soil and water. Exposure to physical hazards associated with the use of machinery in agriculture and forestry: noise, vibration, dust. Prevention of occupational diseases in agriculture, forestry, food industry and wood industry. Work-related accidents and injuries in agriculture, forestry, food industry and wood industry: incidence, causes, social aspects and prevention. State of the health of rural communities depending on various factors: social factors, accessibility of medical care, etc.