The effect of the antioxidant N-acetylcysteine on cholinesterase activity in the brain and blood during Pirimiphos methyl poisoning in the course of treatment with atropine alone, and with atropine and obidoxime.

IF 1.3 4区 医学 Q4 ENVIRONMENTAL SCIENCES
Piotr Adamczuk, Konrad Jamka, Hubert Bojar, Joanna Szala-Rycaj, Aleksandra Szewczyk, Krzysztof Bogumił Sawicki, Grzegorz Raszewski
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Abstract

Introduction and objective: The antioxidant N-acetylcysteine (NAC) may help in the treatment of organophosphates poisoning, including Pirymiphos methyl (PM). However, there is no information on the effect of NAC on target cholinesterases during the core treatment with atropine and obidoxime after acute and chronic exposure to PM. The impact was investigated of NAC on the functional status of target cholinesterases in the brain and blood during treatment with atropine (ATR) and/or obidoxime (OBID) in PM-induced toxicity.

Material and methods: All experiments were performed on Male Swiss mice. The animals were intoxicated with PM and treated with OBID and/or ATR with or without and NAC, in various combinations (with 2-3 drugs) used simultaneously after intoxication. Total acetylcholinesterase activity (AChE) in brain and blood and plasma butyrylcholinesterase activity (BChE) were monitored at 2 and 72 h after intoxication. Enzyme activity was determined using Ellman's colorimetric method.

Results: The applied therapies with OBID, ATR and NAC in various configurations significantly reactivated PM-inhibited AChE in the brain and erythrocytes and the BChE in the plasma. The benefits of NAC administration in combination with ATR and/or OBID therapy have also been reported to restore AChE activity in the brain. NAC may reduce the dose of ATR in the treatment of PM poisoning.

Conclusions: Adjunctive treatment offered by NAC can reduce or prevent the deleterious effects against PM-induced toxicity. Therefore, NAC remains a strong candidate for adjunct treatment for OP-poisoning, including PM, although additional preclinical and clinical studies are needed.

抗氧化剂n -乙酰半胱氨酸对阿托品单用和阿托品与奥比肟联用甲基吡米磷中毒过程中脑和血胆碱酯酶活性的影响
简介和目的:抗氧化剂n -乙酰半胱氨酸(NAC)可能有助于治疗有机磷中毒,包括甲基吡米磷(PM)。然而,在急性和慢性暴露于PM后,在阿托品和奥比肟的核心治疗期间,NAC对目标胆碱酯酶的影响尚无信息。研究了NAC在阿托品(ATR)和/或奥比肟(OBID)治疗pm诱导毒性期间对脑和血液中目标胆碱酯酶功能状态的影响。材料和方法:所有实验均在雄性瑞士小鼠身上进行。动物以PM中毒,中毒后以不同的组合(2-3种药物)同时使用OBID和/或ATR(含或不含NAC)。在中毒后2 h和72 h监测脑和血总乙酰胆碱酯酶活性(AChE)和血浆丁基胆碱酯酶活性(BChE)。用Ellman比色法测定酶活性。结果:不同构型的OBID、ATR和NAC均能显著激活pm,抑制脑和红细胞中的AChE及血浆中的BChE。NAC联合ATR和/或OBID治疗的益处也被报道恢复脑内AChE活性。NAC可减少ATR治疗PM中毒的剂量。结论:NAC辅助治疗可减轻或预防pm引起的毒性反应。因此,尽管还需要进一步的临床前和临床研究,NAC仍然是op中毒(包括PM)辅助治疗的有力候选药物。
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来源期刊
Annals of Agricultural and Environmental Medicine
Annals of Agricultural and Environmental Medicine 医学-公共卫生、环境卫生与职业卫生
CiteScore
3.00
自引率
5.90%
发文量
58
审稿时长
4-8 weeks
期刊介绍: All papers within the scope indicated by the following sections of the journal may be submitted: Biological agents posing occupational risk in agriculture, forestry, food industry and wood industry and diseases caused by these agents (zoonoses, allergic and immunotoxic diseases). Health effects of chemical pollutants in agricultural areas , including occupational and non-occupational effects of agricultural chemicals (pesticides, fertilizers) and effects of industrial disposal (heavy metals, sulphur, etc.) contaminating the atmosphere, soil and water. Exposure to physical hazards associated with the use of machinery in agriculture and forestry: noise, vibration, dust. Prevention of occupational diseases in agriculture, forestry, food industry and wood industry. Work-related accidents and injuries in agriculture, forestry, food industry and wood industry: incidence, causes, social aspects and prevention. State of the health of rural communities depending on various factors: social factors, accessibility of medical care, etc.
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