Tuane Cristine R G Vieira, Breno José Alencar Pires Barbosa
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引用次数: 0
Abstract
Prion diseases, a group of fatal neurodegenerative disorders, present significant diagnostic challenges due to their subtle clinical manifestations, which often mimic those of other treatable neurological conditions. This diagnostic ambiguity underscores the necessity for reliable and specific diagnostic tools. Real-Time Quaking-Induced Conversion (RT-QuIC) has emerged as a groundbreaking technique for diagnosing these diseases using cerebrospinal fluid (CSF) samples. This chapter outlines a comprehensive and standardized protocol for employing RT-QuIC to detect the pathological form of the prion protein in CSF, offering a powerful tool for clinical diagnosis. It provides step-by-step instructions for patient examinations, laboratory RT-QuIC analysis, and data interpretation. By adhering to these protocols, researchers and clinicians can achieve high sensitivity and specificity in diagnosing prion diseases, facilitating timely and accurate patient management.
期刊介绍：
For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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