Zigui-Changqi decoction against premature ovarian insufficiency by moderating autophagy via EGFR/ERBB2/IL6/AKT1 signals.

IF 2 4区医学 Q2 OBSTETRICS & GYNECOLOGY

Gynecologic and Obstetric Investigation Pub Date : 2025-03-28 DOI:10.1159/000545205

Yiwen Wang, Sai Kong, Yanping Qian, Dongqiong Chen, Mengqiu Shao, Manyin Zhai, Lijuan Jiang

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引用次数: 0

Abstract

Background: Autophagy defect has been detected in premature ovarian insufficiency (POI) patients.

Aim: To evaluate the efficacy and explore the mechanism of Zigui-Changqi Decoction (ZCD) in the treatment of POI.

Material and methods: Rat model of POI was established to evaluate the efficacy of ZCD on serum hormone levels, ovarian index, and ovarian cell apoptosis. The effect of ZCD on the autophagy of granulosa cell was assessed. Network pharmacology was used to predict potential targets of ZCD against POI.

Results: ZCD attenuated tripterygium glycosides-induced damage to ovarian tissues in rats by improving ovarian index, moderating serum hormone levels, inhibiting ovarian cell apoptosis, and increasing the number of ovarian stromal cells. ZCD moderated the expression of autophagy-related markers, including BECN1, p62 and LC3. Through network pharmacological analysis, the predicted hub genes were EGFR, ERBB2, AKT1, IL6, PTEN, and TP53. After molecular docking and detection of cell expression levels, EGFR, ERBB2, AKT1, and IL6 were inhibited by ZCD, as well as mTORC1.

Conclusion: ZCD can ameliorate POI, maybe by moderating autophagy via EGFR/ERBB2/IL6/AKT1 signals.

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紫归菖气汤通过EGFR/ERBB2/IL6/AKT1信号调节自噬作用对抗卵巢早衰。

背景：早卵巢功能不全（POI）患者存在自噬缺陷。目的：评价紫桂菖气汤治疗肺水肿的疗效并探讨其作用机制。材料与方法：建立POI大鼠模型，观察ZCD对血清激素水平、卵巢指数及卵巢细胞凋亡的影响。观察ZCD对颗粒细胞自噬的影响。利用网络药理学预测ZCD对POI的潜在靶点。结果：ZCD通过改善卵巢指数、调节血清激素水平、抑制卵巢细胞凋亡、增加卵巢间质细胞数量，减轻雷公藤多苷对大鼠卵巢组织的损伤。ZCD可调节自噬相关标志物BECN1、p62和LC3的表达。通过网络药理学分析，预测中心基因为EGFR、ERBB2、AKT1、IL6、PTEN和TP53。分子对接后检测细胞表达水平，ZCD和mTORC1抑制EGFR、ERBB2、AKT1、IL6。结论：ZCD可能通过EGFR/ERBB2/IL6/AKT1信号调节自噬，从而改善POI。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gynecologic and Obstetric Investigation 医学-妇产科学

CiteScore

4.20

自引率

4.80%

发文量

审稿时长

6-12 weeks

期刊介绍： This journal covers the most active and promising areas of current research in gynecology and obstetrics. Invited, well-referenced reviews by noted experts keep readers in touch with the general framework and direction of international study. Original papers report selected experimental and clinical investigations in all fields related to gynecology, obstetrics and reproduction. Short communications are published to allow immediate discussion of new data. The international and interdisciplinary character of this periodical provides an avenue to less accessible sources and to worldwide research for investigators and practitioners.