Genome-wide identification of NAC transcription factors in Acer paxii, and their expression dynamics during leaf aging.

IF 1.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Genes & genomics Pub Date : 2025-04-01 DOI:10.1007/s13258-025-01638-7

Yuzhi Fei, Shah Faheem Afzal, Zhu Chen, Yue Zhao, Xin Meng, Jie Ren, Shuiming Zhang

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引用次数: 0

Abstract

Background: The NAC family (consisting of NAM, ATAF1/2, and CUC2) represents a crucial plant-specific transcription factor family, contributing significantly to various aspects of plant growth, development, and reactions to abiotic stresses. Yet, the underlying mechanism of NAC regulation in Acer paxii has not been discussed.

Objectives: Identification of NAC genes (ApNACs) in the genome of Acer paxii and exploring the regulatory network of NACs in mediating leaf senescence.

Methods: A thorough genome-wide analysis of the NAC gene family in the Acer paxii genome was performed.

Results: We identified 117 ApNACs from the Acer paxii genome database, which were irregularly distributed across 13 chromosomes. Phylogenetic analysis revealed that ApNAC genes were partitioned into 16 subgroups. There are four kinds of cis-regulatory elements in the promoter region of the ApNACs gene. We compared the expression levels of ApNAC genes at different times using transcriptome data and selected six ApNAC genes for qRT-PCR, which the results showed basic consistency with the transcriptome results. Six ApNACs and 187 TFs from different families were selected, and it was found that the TF families with the highest correlation were WRKY, MYB, bZIP, and ERF, and these TF families were reported to participate in the regulation function in senescence.

Conclusion: This study provides important data support for identifying the NAC gene family of Acer paxii and the regulatory function of the ApNAC genes on plant senescence, which will help to understand the NAC-mediated regulatory network in Acer paxii.

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在全基因组范围内鉴定红叶槭中的 NAC 转录因子及其在叶片老化过程中的表达动态。

背景：NAC家族（由NAM、ATAF1/2和CUC2组成）是一个重要的植物特异性转录因子家族，在植物生长、发育和对非生物胁迫的反应中发挥着重要作用。然而，NAC在槭中调控的潜在机制尚未讨论。目的：鉴定paxii槭（Acer paxii）基因组中的NAC基因（ApNACs），并探索NACs介导叶片衰老的调控网络。方法：对paxii槭NAC基因家族进行全基因组分析。结果：从paxii槭基因组数据库中鉴定出117个ApNACs，这些ApNACs不规则分布在13条染色体上。系统发育分析表明，ApNAC基因可划分为16个亚群。ApNACs基因的启动子区有四种顺式调控元件。我们利用转录组数据比较了ApNAC基因在不同时间的表达水平，选择了6个ApNAC基因进行qRT-PCR，结果与转录组结果基本一致。从不同家族中选取6个ApNACs和187个TF，发现相关性最高的TF家族为WRKY、MYB、bZIP和ERF，据报道这些TF家族参与了衰老的调控功能。结论：本研究为确定paxii槭NAC基因家族及ApNAC基因对植物衰老的调控功能提供了重要的数据支持，有助于了解paxii槭NAC介导的调控网络。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genes & genomics 生物-生化与分子生物学

CiteScore

3.70

自引率

4.80%

发文量

131

审稿时长

6-12 weeks

期刊介绍： Genes & Genomics is an official journal of the Korean Genetics Society (http://kgenetics.or.kr/). Although it is an official publication of the Genetics Society of Korea, membership of the Society is not required for contributors. It is a peer-reviewed international journal publishing print (ISSN 1976-9571) and online version (E-ISSN 2092-9293). It covers all disciplines of genetics and genomics from prokaryotes to eukaryotes from fundamental heredity to molecular aspects. The articles can be reviews, research articles, and short communications.